Mouse anti Human CD45RA:FITC/CD8:RPE has been developed to allow the simulatneous evaluation of CD45RA and CD8 expressing cells by flow cytometry.
CloneF8-11-13 recognises CD45RA, a ~220 kDa glycoprotein expressed by B cells, monocytes and a subset of T-cells.Clone LT8 recognises CD8, a 32kD glycoprotein expressed by the suppressor/cytotoxic subset of T cells, and weakly by NK cells.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
transmembrane receptor protein tyrosine kinase signaling pathway
integral to plasma membrane
antigen processing and presentation
T cell receptor complex
MHC class I protein binding
regulation of immune response
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
immunoglobulin biosynthetic process
transmembrane receptor protein tyrosine phosphatase activity
negative regulation of protein kinase activity
protein kinase binding
positive regulation of B cell proliferation
regulation of S phase
B cell proliferation
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RA/CD8
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Negative Isotype Controls Available
Useful Reagents Available