Mouse anti Human CD45RA:FITC/CD8:RPE has been developed to allow the simulatneous evaluation of CD45RA and CD8 expressing cells by flow cytometry.
CloneF8-11-13 recognises CD45RA, a ~220 kDa glycoprotein expressed by B cells, monocytes and a subset of T-cells.Clone LT8 recognises CD8, a 32kD glycoprotein expressed by the suppressor/cytotoxic subset of T cells, and weakly by NK cells.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
transmembrane receptor protein tyrosine kinase signaling pathway
integral to plasma membrane
antigen processing and presentation
T cell receptor complex
MHC class I protein binding
regulation of immune response
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
immunoglobulin biosynthetic process
transmembrane receptor protein tyrosine phosphatase activity
negative regulation of protein kinase activity
protein kinase binding
positive regulation of B cell proliferation
regulation of S phase
B cell proliferation
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RA/CD8
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
How to Use the Spectraviewer
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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra