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CD45RA/CD8 | F8-11-13 / LT8

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Mouse anti Human CD45RA:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
F8-11-13 / LT8
Isotype
Cocktail
Specificity
CD45RA/CD8

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Mouse anti Human CD45RA:FITC/CD8:RPE has been developed to allow the simulatneous evaluation of CD45RA and CD8 expressing cells by flow cytometry.

CloneF8-11-13 recognises CD45RA, a ~220 kDa glycoprotein expressed by B cells, monocytes and a subset of T-cells.Clone LT8 recognises CD8, a 32kD glycoprotein expressed by the suppressor/cytotoxic subset of T cells, and weakly by NK cells.

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC012 F 50 Tests loader
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Description Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

RRID
AB_1125265
UniProt
P01732
P08575
P10966
Entrez Gene
CD8A
PTPRC
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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