CD45RA/CD8 | F8-11-13 / LT8

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Mouse anti Human CD45RA:FITC/CD8:RPE

Product Type: Dual Color Reagent
Clone: F8-11-13 / LT8
Isotype: Cocktail
Specificity: CD45RA/CD8

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DC010 Datasheet Datasheet SDS SDS	F	50 Tests		Log in	Get Quote
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Mouse anti Human CD45RA:FITC/CD8:RPE has been developed to allow the simulatneous evaluation of CD45RA and CD8 expressing cells by flow cytometry.

CloneF8-11-13 recognises CD45RA, a ~220 kDa glycoprotein expressed by B cells, monocytes and a subset of T-cells.Clone LT8 recognises CD8, a 32kD glycoprotein expressed by the suppressor/cytotoxic subset of T cells, and weakly by NK cells.

Target Species: Human
Product Form: Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution: Reconstitute with 0.5 ml distilled water
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Antibody Isotypes: FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525

RPE 488nm laser 496 578

RPE 561nm laser 546 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
RPE 488nm laser	496	578
RPE 561nm laser	546	578

Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control	DC012	F	50 Tests	Log in	Get Quote
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Description	Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control

Description	Product Code	Applications	Pack Size	Your Price
Human Seroblock	BUF070A	F	50 Test	Log in	Get Quote
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Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test	Log in	Get Quote
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Description	Human Seroblock

RRID: AB_1125265
UniProt: P01732; P08575; P10966
Entrez Gene: CD8A; PTPRC; CD8B
GO Terms: GO:0005576 extracellular region; GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway; GO:0005887 integral to plasma membrane; GO:0015026 coreceptor activity; GO:0019882 antigen processing and presentation; GO:0042101 T cell receptor complex; GO:0042288 MHC class I protein binding; GO:0050776 regulation of immune response; GO:0001915 negative regulation of T cell mediated cytotoxicity; View More GO Terms; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0002378 immunoglobulin biosynthetic process; GO:0005001 transmembrane receptor protein tyrosine phosphatase activity; GO:0005925 focal adhesion; GO:0006469 negative regulation of protein kinase activity; GO:0006470 protein dephosphorylation; GO:0007411 axon guidance; GO:0019901 protein kinase binding; GO:0030890 positive regulation of B cell proliferation; GO:0033261 regulation of S phase; GO:0042100 B cell proliferation; GO:0045121 membrane raft; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0050857 positive regulation of antigen receptor-mediated signaling pathway; GO:0051209 release of sequestered calcium ion into cytosol; GO:0051607 defense response to virus