CD45RA/CD45RO/CD8/CD3 | F8-11-13 / UCHL1 / LT8 / UCHT1

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Four-Color Human CD45RA/CD45RO/CD8/CD3 Flow Kit

Product Type
Four Color Reagent
Clone
F8-11-13 / UCHL1 / LT8 / UCHT1
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4C005 F 50 Tests/0.5ml
Four-Color Human CD45RA/CD45RO/CD8/CD3 Flow Kit can be used for single-step identification of human suppressor/cytotoxic naïve (CD3+CD8+CD45RA+) and suppressor/cytotoxic memory (CD3+CD8+CD45RO+) T-cell subsets.

CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain.

CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human suppressor/cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

CD45RA is an isoform of CD45, a member of the protein tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), which acts as a positive regulator of T-cell co-activation, and is essential for the activation of T-cells through the antigen receptor. CD45RA is expressed by naïve T-cells, but not by activated and memory T-cells. Consequently, clone F8-11-13 can be used to distinguish between these cell populations.

CD45RO is the low molecular weight isoform (~180 kDa) of CD45, the leucocyte common antigen (LCA). CD45RO is expressed by approximately 40% of resting peripheral blood T-cells, the majority of activated and memory T-cells and most thymocytes. It is also expressed on macrophages, monocytes and granulocytes. In combination with clone F8-11-13, clone UCHL1 can be used to discern naïve and memory T-cells.

Product Details

Target Species
Human
Product Form
Four color combination consisting of FITC, RPE, APC and RPE-Cy5.5 conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

FITC conjugated CD45RA (Mouse IgG1)
RPE conjugated CD45RO (Mouse IgG2a)
APC conjugated CD8 (Mouse IgG1)
RPE-Cy5.5 conjugated CD3 (Mouse IgG1)
Reconstitution
Reconstitute with 0.5ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08575 Related reagents
P07766 Related reagents
P01732 Related reagents
Entrez Gene
CD8A Related reagents
CD3E Related reagents
PTPRC Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD45RA/CD45RO/CD8/CD3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04B 100 Test/20ml F
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04C 500 Tests/100ml F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Flow Cytometry Absolute Count Standard™ FCSC580 20 Tests F

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