CD45 antibody | OTI1H2

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Anti CD45 Antibody, clone OTI1H2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CD45 Antibody, clone OTI1H2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human CD45 antibody, clone OTI1H2 (**VMA00169**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to CD45 (molecular weight 240 kDa).

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Mouse anti CD45

Product Type: PrecisionAb Monoclonal
Clone: OTI1H2
Isotype: IgG1
Format: Purified
Specificity: CD45

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00169 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human CD45 antibody, clone OTI1H2 recognizes CD45, also known as T200 glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide and receptor-type tyrosine-protein phosphatase C.

CD45, encoded by the PTPRC gene, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of PTPRC, which encode distinct isoforms, have been reported (provided by RefSeq, Jun 2012).

Mouse anti Human CD45 antibody detects a band of 240 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CD45 detects a band of approximately 240 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human CD45 (NP_002829) produced in HEK293T cells.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P08575
Entrez Gene: PTPRC
GO Terms: GO:0001915 negative regulation of T cell mediated cytotoxicity; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0002378 immunoglobulin biosynthetic process; GO:0005001 transmembrane receptor protein tyrosine phosphatase activity; GO:0005887 integral to plasma membrane; GO:0005925 focal adhesion; GO:0006469 negative regulation of protein kinase activity; GO:0006470 protein dephosphorylation; GO:0007411 axon guidance; View More GO Terms; GO:0019901 protein kinase binding; GO:0030890 positive regulation of B cell proliferation; GO:0033261 regulation of S phase; GO:0042100 B cell proliferation; GO:0045121 membrane raft; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0050857 positive regulation of antigen receptor-mediated signaling pathway; GO:0051209 release of sequestered calcium ion into cytosol; GO:0051607 defense response to virus