CD44v4 antibody | VFF-11
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|Mouse anti Human CD44v4 antibody, clone VFF-11 recognizes an epitope encoded by exon v4 on the variant portion of human CD44. CD44v4 is strongly expressed on some breast cancer cell lines, notably epithelial-like BT-20 cells where v4 containing isoforms possess functional E-selectin ligand activity mediating cell adhesion under physiological flow conditions (Shirure et al. 2014).|
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/500|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD44v4 antibody
Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
J Exp Med. 177 (4): 897-904.
Hanley, W.D. et al. (2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
FASEB J. 20: 337-9.
Rajarajan, A. et al. (2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.
PLoS One. 7: e28776.
Chandrasekaran, S. et al. (2012) Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines
Biomaterials. 33: 9037-48.
El-Sharkawy, M.M. et al. (2003) CD44 expression and soluble CD44 as a potential marker of tumor load in pediatric acute leukemia.
J Egypt Nat Cancer Inst. 15: 129-35.
Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
Am J Physiol Cell Physiol. 308 (1): C68-78.
Hudson, D.L. et al. (1995) CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion.
J Cell Sci. 108: 1959-70.
De Sousa, P. A. (2009) Method for differentiation of stem cells.
U.S. Patent Application: US20090123430 A1
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Lallana, E. et al. (2017) Chitosan/Hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA Delivery.
Mol Pharm. 14 (7): 2422-36.
Spadea, A. et al. (2019) Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
Mol Pharm. 16 (6): 2481-93.
Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
Integr Biol (Camb). 10 (12): 747-57.
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