CD44v4 antibody | VFF-11
Detection of total CD44 and variant CD44 protein. Representative figures of immunostaining with all cells stained positive in this field (A), western blotting (B), and flow cytometry (C). (A) Immunostaining of HN12 cells for total CD44, an isotype matched control, v3, v4, v5, and v6. (B) Western blotting lanes 1–12 contain MW marker, HaCaT, HN4, HN12, HN8, HN22, HN30, HN31, OSC-19, OSC-20, DT, and Namalwa cells, repectively. (C) flow cytometry results for HN4 and HN12 cells detecting v7 and v10.
From: Rajarajan A, Stokes A, Bloor BK, Ceder R, Desai H, et al. (2012) CD44 Expression in Oro-Pharyngeal Carcinoma Tissues and Cell Lines. PLoS ONE 7(1): e28776.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44v4 antibody, clone VFF-11 recognizes an epitope encoded by exon v4 on the variant portion of human CD44. CD44v4 is strongly expressed on some breast cancer cell lines, notably epithelial-like BT-20 cells where v4 containing isoforms possess functional E-selectin ligand activity mediating cell adhesion under physiological flow conditions (Shirure et al. 2014).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Tris buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.01% Thiomersal - Immunogen
- Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD44v4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | ||
| Immunohistology - Frozen | 1/50 | 1/500 | |
| Immunohistology - Paraffin 1 | |||
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD44v4 antibody
-
Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
J Exp Med. 177 (4): 897-904. -
Hanley, W.D. et al. (2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
FASEB J. 20: 337-9. -
Rajarajan, A. et al. (2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.
PLoS One. 7: e28776. -
Chandrasekaran, S. et al. (2012) Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines
Biomaterials. 33: 9037-48. -
El-Sharkawy, M.M. et al. (2003) CD44 expression and soluble CD44 as a potential marker of tumor load in pediatric acute leukemia.
J Egypt Nat Cancer Inst. 15: 129-35. -
Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
Am J Physiol Cell Physiol. 308 (1): C68-78. -
Hudson, D.L. et al. (1995) CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion.
J Cell Sci. 108: 1959-70. -
De Sousa, P. A. (2009) Method for differentiation of stem cells.
U.S. Patent Application: US20090123430 A1 -
Lallana, E. et al. (2017) Chitosan/Hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA Delivery.
Mol Pharm. 14 (7): 2422-36. -
Spadea, A. et al. (2019) Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
Mol Pharm. 16 (6): 2481-93. -
Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
Integr Biol (Camb). 10 (12): 747-57.
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