CD44v4 antibody | VFF-11

Mouse anti Human CD44v4

Product Type
Monoclonal Antibody
Clone
VFF-11
Isotype
IgG1
Specificity
CD44v4

Product Code Applications Pack Size List Price Your Price Qty
MCA1728
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.1 mg loader
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loader
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Mouse anti Human CD44v4 antibody, clone VFF-11 recognizes an epitope encoded by exon v4 on the variant portion of human CD44. CD44v4 is strongly expressed on some breast cancer cell lines, notably epithelial-like BT-20 cells where v4 containing isoforms possess functional E-selectin ligand activity mediating cell adhesion under physiological flow conditions (Shirure et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50
Immunohistology - Frozen 1/50 1/500
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD44v4 antibody

  1. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
    J Exp Med. 177 (4): 897-904.
  2. Hanley, W.D. et al. (2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
    FASEB J. 20: 337-9.
  3. Rajarajan, A. et al. (2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.
    PLoS One. 7: e28776.
  4. Chandrasekaran, S. et al. (2012) Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines
    Biomaterials. 33: 9037-48.
  5. El-Sharkawy, M.M. et al. (2003) CD44 expression and soluble CD44 as a potential marker of tumor load in pediatric acute leukemia.
    J Egypt Nat Cancer Inst. 15: 129-35.
  6. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
    Am J Physiol Cell Physiol. 308 (1): C68-78.
  7. Hudson, D.L. et al. (1995) CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion.
    J Cell Sci. 108: 1959-70.
  8. De Sousa, P. A. (2009) Method for differentiation of stem cells.
    U.S. Patent Application: US20090123430 A1
  9. View The Latest Product References
  10. Lallana, E. et al. (2017) Chitosan/Hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA Delivery.
    Mol Pharm. 14 (7): 2422-36.
  11. Spadea, A. et al. (2019) Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
    Mol Pharm. 16 (6): 2481-93.
  12. Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
    Integr Biol (Camb). 10 (12): 747-57.

Flow Cytometry

Western Blotting

RRID
AB_322690
UniProt
P16070
Entrez Gene
CD44
View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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