CD44 antibody
Mouse anti CD44
CD44 antibody recognizes CD44 antigen, also known as CDw44, epican, extracellular matrix receptor III, ECMR-III, HUTCH-I, phagocytic glycoprotein 1, PGP-1, phagocytic glycoprotein I, PGP-I or CD44. The antibody has been extensively validated for western blotting using whole cell lysates.
CD44 Antibody with Control Lysate
CD44 antibody recognizes CD44 antigen, also known as CDw44, epican, extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, heparan sulfate proteoglycan, hermes antigen, hyaluronate receptor, phagocytic glycoprotein 1, PGP-1, or CD44.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00034 | WB | 100 µl | ||||
| VMA00034KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CD44 antibody recognizes human CD44, a 742 amino acid, type I transmembrane protein which is otherwise known as HCAM, epican or CD44s. Multiple isoforms of CD44 exist, generated by alternative splicing within a single variable extracellular domain (Jackson et al. 1992). The standard form of CD44, recognized by Mouse anti Human CD44 antibody, is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Mouse anti Human antibody binds to a protease resistant epitope on the CD44 molecule.
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (Hofmann et al. 1991).
Mouse anti Human CD44 antibody recognizes the standard CD44 isoform lacking any of the variable region exon products as a ~82 kDa band in HeLa cell line lysates by western blotting under reducing conditions. In addition, Mouse anti Human CD44 antibody will recognize all higher molecular weight forms of CD44 containing one or more of the variable region exon products.
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (Hofmann et al. 1991).
Mouse anti Human CD44 antibody recognizes the standard CD44 isoform lacking any of the variable region exon products as a ~82 kDa band in HeLa cell line lysates by western blotting under reducing conditions. In addition, Mouse anti Human CD44 antibody will recognize all higher molecular weight forms of CD44 containing one or more of the variable region exon products.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CD44 antibody detects a band of approximately 82 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Stimulated human leucocytes
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P16070 Related reagents
- Entrez Gene
- CD44 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CD44 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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