CD44 antibody | 156-3C11

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Mouse anti CD44

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human CD44 antibody recognizes human CD44, a 742 amino acid, type I transmembrane protein which is otherwise known as HCAM, epican or CD44s. Multiple isoforms of CD44 exist, generated by alternative splicing within a single variable extracellular domain (Jackson et al. 1992). The standard form of CD44, recognized by Mouse anti Human CD44 antibody, is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Mouse anti Human antibody binds to a protease resistant epitope on the CD44 molecule.

CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (Hofmann et al. 1991).

Mouse anti Human CD44 antibody recognizes the standard CD44 isoform lacking any of the variable region exon products as a ~82 kDa band in HeLa cell line lysates by western blotting under reducing conditions. In addition, Mouse anti Human CD44 antibody will recognize all higher molecular weight forms of CD44 containing one or more of the variable region exon products.

Product Details

Target Species
Western Blotting
Anti CD44 antibody detects a band of approximately 82 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Stimulated human leucocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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