CD41/CD61 antibody | A10-33/1

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Mouse anti Human CD41/CD61

Product Type: Monoclonal Antibody
Clone: A10-33/1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
4108-7204	C* WB			0.5 ml

Mouse anti Human CD41/CD51 antibody, clone A10-33/1 recognizes endothelial cells in various cultures and organs (including lung, liver, gall bladder and skin). It is generally reactive with melanoma, including metastasizing melanomas and in carcinomas. It reacts strongly with purified GPIIb-IIIa which is expressed on platelets but not endothelial cells. Cross reactivity with nervous tissue has also been reported.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - lyophilised

Reconstitution

Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Purified IgG prepared by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal
1.0% Bovine Serum Albumin

Immunogen

Human melanoma cell lines.

Approx. Protein Concentrations

IgG concentration 0.12 mg/ml after reconstitution.

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P08514 Related reagents; P05106 Related reagents
Entrez Gene: ITGB3 Related reagents; ITGA2B Related reagents
GO Terms: GO:0001938 positive regulation of endothelial cell proliferation; GO:0002576 platelet degranulation; GO:0050839 cell adhesion molecule binding; GO:0004872 receptor activity; GO:0005161 platelet-derived growth factor receptor binding; GO:0008305 integrin complex; GO:0007160 cell-matrix adhesion; GO:0007229 integrin-mediated signaling pathway; GO:0007411 axon guidance; GO:0042802 identical protein binding; GO:0010595 positive regulation of endothelial cell migration; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010888 negative regulation of lipid storage; GO:0014909 smooth muscle cell migration; GO:0030168 platelet activation; GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway; GO:0031092 platelet alpha granule membrane; GO:0032147 activation of protein kinase activity; GO:0032369 negative regulation of lipid transport; GO:0035295 tube development; GO:0043184 vascular endothelial growth factor receptor 2 binding; GO:0044419 interspecies interaction between organisms; GO:0045124 regulation of bone resorption; GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050748 negative regulation of lipoprotein metabolic process; GO:0050900 leukocyte migration; GO:0060055 angiogenesis involved in wound healing; GO:0071062 alphav-beta3 integrin-vitronectin complex
Regulatory: For research purposes only

Applications of CD41/CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen ¹	1/100	1/400
Immunohistology - Paraffin
Western Blotting

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human Placenta

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for CD41/CD61 antibody

Brüggen, J. & Sorg, C. (1983) Detection of phenotypic differences on human malignant melanoma lines and their variant sublines with monoclonal antibodies.
Cancer Immunol Immunother. 15 (3): 200-5.

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