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CD41/CD61 antibody | A10-33/1

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Mouse anti Human CD41/CD61

Product Type
Monoclonal Antibody
Clone
A10-33/1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4108-7204 C* WB 0.5 ml
Mouse anti Human CD41/CD51 antibody, clone A10-33/1 recognizes endothelial cells in various cultures and organs (including lung, liver, gall bladder and skin). It is generally reactive with melanoma, including metastasizing melanomas and in carcinomas. It reacts strongly with purified GPIIb-IIIa which is expressed on platelets but not endothelial cells. Cross reactivity with nervous tissue has also been reported.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
1.0% Bovine Serum Albumin
Immunogen
Human melanoma cell lines.
Approx. Protein Concentrations
IgG concentration 0.12 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08514 Related reagents
P05106 Related reagents
Entrez Gene
ITGB3 Related reagents
ITGA2B Related reagents
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002576 platelet degranulation
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0010595 positive regulation of endothelial cell migration
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0014909 smooth muscle cell migration
GO:0030168 platelet activation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0031092 platelet alpha granule membrane
GO:0032147 activation of protein kinase activity
GO:0032369 negative regulation of lipid transport
GO:0035295 tube development
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050748 negative regulation of lipoprotein metabolic process
GO:0050900 leukocyte migration
GO:0060055 angiogenesis involved in wound healing
GO:0071062 alphav-beta3 integrin-vitronectin complex
Regulatory
For research purposes only

Applications of CD41/CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/100 1/400
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Placenta
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for CD41/CD61 antibody

  1. Brüggen, J. & Sorg, C. (1983) Detection of phenotypic differences on human malignant melanoma lines and their variant sublines with monoclonal antibodies.
    Cancer Immunol Immunother. 15 (3): 200-5.
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