Mouse anti Human CD41/CD51 antibody, clone A10-33/1 recognizes endothelial cells in various cultures and organs (including lung, liver, gall bladder and skin). It is generally reactive with melanoma, including metastasizing melanomas and in carcinomas. It reacts strongly with purified GPIIb-IIIa which is expressed on platelets but not endothelial cells. Cross reactivity with nervous tissue has also been reported.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
1.0% Bovine Serum Albumin
- Human melanoma cell lines.
- Approx. Protein Concentrations
- IgG concentration 0.12 mg/ml after reconstitution.
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
positive regulation of endothelial cell proliferation
cell adhesion molecule binding
platelet-derived growth factor receptor binding
integrin-mediated signaling pathway
identical protein binding
positive regulation of endothelial cell migration
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
smooth muscle cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
platelet alpha granule membrane
activation of protein kinase activity
negative regulation of lipid transport
vascular endothelial growth factor receptor 2 binding
interspecies interaction between organisms
regulation of bone resorption
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of lipoprotein metabolic process
angiogenesis involved in wound healing
alphav-beta3 integrin-vitronectin complex
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD41/CD61 antibody
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Placenta
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Secondary Antibodies Available
Product Specific References
References for CD41/CD61 antibody
Brüggen, J. & Sorg, C. (1983) Detection of phenotypic differences on human malignant melanoma lines and their variant sublines with monoclonal antibodies.
Cancer Immunol Immunother. 15 (3): 200-5.