Anti Human CD4:FITC/CD8:RPE/CD3:RPE-CY5 may be used to simultaneously identify CD3, CD4 and CD8 positive lymphocytes.
Clone RPA-T4 is specific for the human CD4 antigen, a 59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T-cells and weakly by monocytes. LT8 recognizes the human CD8 antigen, a 32 kDa dimer expressed by a subset of T-cells with cytotoxic/suppressor activity and more weakly by NK cells. Clone S4.1 is specific for the human CD3 antigen, a pan T cell marker.
- Target Species
- Product Form
- Triple Color combination consisting of FITC, RPE and RPE-CY5 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- CD4 FITC reagent: Mouse IgG1
CD8 RPE reagent: Mouse IgG1
CD3 RPE-CY5 reagent: Mouse IgG2a
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD4/CD8/CD3
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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