CD4/CD8/CD3 | RPA-T4 / LT8 / S4.1

anti Human CD4:FITC/CD8:RPE/CD3:RPE-Cy5

Product Type
Triple Color Reagent
RPA-T4 / LT8 / S4.1

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 50 Tests loader
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Search for Batch Specific Datasheets

Anti Human CD4:FITC/CD8:RPE/CD3:RPE-CY5 may be used to simultaneously identify CD3, CD4 and CD8 positive lymphocytes.

Clone RPA-T4 is specific for the human CD4 antigen, a 59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T-cells and weakly by monocytes. LT8 recognizes the human CD8 antigen, a 32 kDa dimer expressed by a subset of T-cells with cytotoxic/suppressor activity and more weakly by NK cells. Clone S4.1 is specific for the human CD3 antigen, a pan T cell marker.

Target Species
Product Form
Triple Color combination consisting of FITC, RPE and RPE-CY5 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Antibody Isotypes
CD4 FITC reagent: Mouse IgG1
CD8 RPE reagent: Mouse IgG1
CD3 RPE-CY5 reagent: Mouse IgG2a
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
RPE-Cy5 488nm laser 496 667
RPE-Cy5 561nm laser 546 667
For research purposes only
12 months from date of despatch

Store at +4oC.


This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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