CD4/CD25 | 7E14/1TYV

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Mouse anti Human CD4:FITC/CD25:RPE

Product Type
Dual Color Reagent
Clone
7E14/1TYV
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC027 F 50 Tests
Mouse anti Human CD4:FITC/CD25:RPE Dual color reagent is designed for the simultaneous enumeration of CD4 and CD25 positive cells, and is particularly useful for the assessment of the activation status of CD4+ve T cells.

Product Details

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
0.2%Gelatin
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P01730 Related reagents
P01589 Related reagents
Entrez Gene
IL2RA Related reagents
CD4 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004911 interleukin-2 receptor activity
GO:0008283 cell proliferation
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CD4/CD25

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 106 cells or 100ul whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC012 50 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F