CD4 antibody | YNB46.1.8

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Rat anti Human CD4

Product Type
Monoclonal Antibody
Clone
YNB46.1.8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA484GT C F 25 µg
MCA484G C F 0.2 mg
Rat anti Human CD4 antibody, clone YNB46.1.8 recognizes the human CD4 cell surface antigen, a 55 kDa molecule expressed by helper T lymphocytes and by monocytes.

Rat anti Human CD4 antibody, clone YNB46.1.8 blocks syncytia formation of U937 cells infected with HIV.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat cells transfected with human CD4.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised PVG-RT1u rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01730 Related reagents
Entrez Gene
CD4 Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using approprite negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA6004GA 0.1 mg F

Application Based External Images

Immunohistology - Frozen

Product Specific References

Source Reference

  1. Mathieson, P.W. et al. (1990) Monoclonal-antibody therapy in systemic vasculitis.
    N Engl J Med. 323 (4): 250-4.

References for CD4 antibody

  1. Hirbod, T. et al. (2010) Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men.
    Am J Pathol. 176: 2798-805.
  2. Yawalkar, N. et al. (2000) T cells isolated from positive epicutaneous test reactions to amoxicillin and ceftriaxone are drug specific and cytotoxic.
    J Invest Dermatol. 115 (4): 647-52.
  3. Markovic, I. et al. (2004) Thiol/disulfide exchange is a prerequisite for CXCR4-tropic HIV-1 envelope-mediated T-cell fusion during viral entry.
    Blood. 103 (5): 1586-94.
  4. Hirbod, T. et al. (2011) In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
    PLoS One. 6 (9): e25551.
  5. Op De Beeck, A. et al. (2004) The transmembrane domains of the prM and E proteins of yellow fever virus are endoplasmic reticulum localization signals.
    J Virol. 78 (22): 12591-602.
  6. Yawalkar, N. et al. (2001) A comparative study of the expression of cytotoxic proteins in allergic contact dermatitis and psoriasis: spongiotic skin lesions in allergic contact dermatitis are highly infiltrated by T cells expressing perforin and granzyme B.
    Am J Pathol. 158 (3): 803-8.