CD36 antibody | SMØ

Product Code Applications Pack Size List Price Your Price Qty
MCA722
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.2 mg loader
List Price Your Price
loader
MCA722GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD36 antibody, clone SMØ recognizes the CD36 antigen, an ~88 kDa cell surface glycoprotein expressed by platelets, monocytes/macrophages and also by some microvascular endothelium and erythroid precursors.

CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages, CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. Mouse anti Human CD36 antibody, clone SMØ partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding, P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team.

Mouse anti Human CD36 antibody, clone SMØ is also reported to block interaction of oxidized low-density lipoprotein (OXLDL) with CD36 (Sugihara et al.1992).

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human tonsil cells and peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the NSI mouse myeloma line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 Pack Size: 0.2 mg
1/10 Pack Size: 0.1 mg
1/100 Pack Size: 0.2 mg
1/20 Pack Size: 0.1 mg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
List Price Your Price
loader
Description Human anti Mouse IgM:FITC
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgM Negative Control

References for CD36 antibody

  1. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
    Immunology. 53 (4): 753-67.
  2. Sarigianni, M. et al. (2010) Effect of leptin and insulin resistance on properties of human monocytes in lean and obese healthy participants.
    Angiology. 61: 768-74.
  3. Sugihara, K. et al. (1992) Thrombospondin mediates adherence of CD36+ sickle reticulocytes to endothelial cells.
    Blood. 80 (10): 2634-42.
  4. Biswas, A.K. et al. (2007) Plasmodium falciparum uses gC1qR/HABP1/p32 as a receptor to bind to vascular endothelium and for platelet-mediated clumping.
    PLoS Pathog. 3: 1271-80.
  5. Pain, A. et al. (2001) Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria.
    Proc Natl Acad Sci U S A. 98: 1805-10.
  6. Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.
    J Cell Physiol. 202: 731-42.

Further Reading

  1. Greenwalt, D.E. et al. (1992) Membrane glycoprotein CD36: a review of its roles in adherence, signal transduction, and transfusion medicine.
    Blood. 80 (5): 1105-15.
  2. Endemann, G. et al. (1993) CD36 is a receptor for oxidized low density lipoprotein.
    J Biol Chem. 268 (16): 11811-6.

Functional Assays

Synonyms
GPIV (IIIb)
RRID
AB_321675
UniProt
P16671
Entrez Gene
CD36
View more products with CD36 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products