Mouse anti Human CD34 antibody, clone 581
recognizes the human CD34 cell surface antigen also known as Hematopoietic progenitor cell antigen CD34. Clone 581 binds to an epitope of human CD34 resistant to neuraminadase, chymopapain and glycoprotease treatments. The binding epitopes are classified according to their resistance to enzymatic degradation and antibodies assigned according to this and cross inhibition studies. Clone 581 is classified as class III (Lanza et al. 1999
CD34 is a 385 amino acid pro-peptide with the 31 amino acid signal peptide cleaved to yield the mature form of CD34. CD34 is a single pass type I ~105-120 kDa highly glycosylated transmembrane glycoprotein, expressed by hematopoietic stem cells and small vessel endothelial cells. CD34 contains a number of sialylated residues and has a mucin-like structure (Simmons et al. 1992
). A truncated form of the CD34 molecule has also been identified lacking the carboxy-terminal region of the intracellular sequence, also bearing a sequence switch in the remaining cytoplasmic region (Nakamura et al. 1993
). Mouse anti Human CD34 antibody, clone 581 is expected to react with both isoforms of the CD34 molecule as it recognizes an epitope located in the extracellular region of human CD34.
Mouse anti Human CD34 antibody, clone 581 has been used successfully for the immunohistochemical detection of stem-like cells present in hepatoblastoma lesions on cryostat sections (Fiegal et al. 2004
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD34 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul