CD34 antibody | 581

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Mouse anti Human CD34:RPE

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
100 Tests loader

Mouse anti Human CD34 antibody, clone 581 recognizes the human CD34 cell surface antigen also known as Hematopoietic progenitor cell antigen CD34. Clone 581 binds to an epitope of human CD34 resistant to neuraminadase, chymopapain and glycoprotease treatments. The binding epitopes are classified according to their resistance to enzymatic degradation and antibodies assigned according to this and cross inhibition studies. Clone 581 is classified as class III (Lanza et al. 1999).

CD34 is a 385 amino acid pro-peptide with the 31 amino acid signal peptide cleaved to yield the mature form of CD34. CD34 is a single pass type I ~105-120 kDa highly glycosylated transmembrane glycoprotein, expressed by hematopoietic stem cells and small vessel endothelial cells. CD34 contains a number of sialylated residues and has a mucin-like structure (Simmons et al. 1992). A truncated form of the CD34 molecule has also been identified lacking the carboxy-terminal region of the intracellular sequence, also bearing a sequence switch in the remaining cytoplasmic region (Nakamura et al. 1993). Mouse anti Human CD34 antibody, clone 581 is expected to react with both isoforms of the CD34 molecule as it recognizes an epitope located in the extracellular region of human CD34.

Mouse anti Human CD34 antibody, clone 581 has been used successfully for the immunohistochemical detection of stem-like cells present in hepatoblastoma lesions on cryostat sections (Fiegal et al. 2004).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin

Storage Information

Store at +4oC.


This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
6 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0030246 carbohydrate binding
GO:0009897 external side of plasma membrane
GO:0016337 cell-cell adhesion
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
For research purposes only

Applications of CD34 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Product Specific References

References for CD34 antibody

  1. Civin, C.I. et al. (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.
    J Immunol. 133 (1): 157-65.
  2. Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
    J Histochem Cytochem. 52 (11): 1495-501.
  3. Maumus, M. et al. (2011) Native human adipose stromal cells: localization, morphology and phenotype.
    Int J Obes (Lond). 35 (9): 1141-53.
  4. Patel, J. et al. (2013) Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta.
    Stem Cells Transl Med. 2 (11): 839-47.
  5. Park, J.Y. et al. (2020) rhBMP-2 Pre-Treated Human Periodontal Ligament Stem Cell Sheets Regenerate a Mineralized Layer Mimicking Dental Cementum.
    Int J Mol Sci. 21 (11): 3767.
  6. Kim, S.H. et al. (2019) Forkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromal cells.
    J Biol Chem. 294 (21): 8424-37.

Further Reading

  1. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp. Anim. 49: 97-110.

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