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CD33 antibody | WM53

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Anti Human CD33 Antibody, clone WM53 thumbnail image 1 Anti Human CD33 Antibody, clone WM53 thumbnail image 2 Anti Human CD33 Antibody, clone WM53 thumbnail image 3 Anti Human CD33 Antibody, clone WM53 thumbnail image 4 Anti Human CD33 Antibody, clone WM53 thumbnail image 5 Anti Human CD33 Antibody, clone WM53 thumbnail image 6 Anti Human CD33 Antibody, clone WM53 thumbnail image 7 Anti Human CD33 Antibody, clone WM53 thumbnail image 8 Anti Human CD33 Antibody, clone WM53 thumbnail image 9 Anti Human CD33 Antibody, clone WM53 thumbnail image 10 Anti Human CD33 Antibody, clone WM53 thumbnail image 11 Anti Human CD33 Antibody, clone WM53 thumbnail image 12 Anti Human CD33 Antibody, clone WM53 thumbnail image 13 Anti Human CD33 Antibody, clone WM53 thumbnail image 14 Anti Human CD33 Antibody, clone WM53 thumbnail image 15 Anti Human CD33 Antibody, clone WM53 thumbnail image 16 Anti Human CD33 Antibody, clone WM53 thumbnail image 17 Anti Human CD33 Antibody, clone WM53 thumbnail image 18 Anti Human CD33 Antibody, clone WM53 thumbnail image 19 Anti Human CD33 Antibody, clone WM53 thumbnail image 20 Anti Human CD33 Antibody, clone WM53 thumbnail image 21 Anti Human CD33 Antibody, clone WM53 thumbnail image 22 Anti Human CD33 Antibody, clone WM53 thumbnail image 23 Anti Human CD33 Antibody, clone WM53 thumbnail image 24 Anti Human CD33 Antibody, clone WM53 thumbnail image 25 Anti Human CD33 Antibody, clone WM53 thumbnail image 26 Anti Human CD33 Antibody, clone WM53 thumbnail image 27 Anti Human CD33 Antibody, clone WM53 thumbnail image 28 Anti Human CD33 Antibody, clone WM53 thumbnail image 29 Anti Human CD33 Antibody, clone WM53 thumbnail image 30 Anti Human CD33 Antibody, clone WM53 thumbnail image 31 Anti Human CD33 Antibody, clone WM53 thumbnail image 32 Anti Human CD33 Antibody, clone WM53 thumbnail image 33 Anti Human CD33 Antibody, clone WM53 thumbnail image 34 Anti Human CD33 Antibody, clone WM53 thumbnail image 35 Anti Human CD33 Antibody, clone WM53 thumbnail image 36 Anti Human CD33 Antibody, clone WM53 thumbnail image 37 Anti Human CD33 Antibody, clone WM53 thumbnail image 38 Anti Human CD33 Antibody, clone WM53 thumbnail image 39 Anti Human CD33 Antibody, clone WM53 thumbnail image 40 Anti Human CD33 Antibody, clone WM53 thumbnail image 41 Anti Human CD33 Antibody, clone WM53 thumbnail image 42 Anti Human CD33 Antibody, clone WM53 thumbnail image 43 Anti Human CD33 Antibody, clone WM53 thumbnail image 44
Anti Human CD33 Antibody, clone WM53 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Amethyst Orange conjugated Mouse anti Human CD33 (MCA1271AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD33 Antibody, clone WM53 gallery image 2
Figure A. FITC conjugated mouse anti human CD14 (MCA596F) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD14 (MCA596F) and Alexa647® conjugated mouse anti human CD33 (MCA1271A647). All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).
Anti Human CD33 Antibody, clone WM53 gallery image 3
Published customer image:
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived NK cells by flow cytometry.
Image caption:
CD33 expression and function in primary human NK cells. (a) Blood NK cells (CD3-CD56+ lymphocytes in region R1) and (b, c) quiescent primary human NK cells were phenotyped by flow cytometry. Blood CD33+ NK cells were mainly CD56bright (R2 region, dark dots), CD16-, CD57-, NKG2A+, and NKG2D+. The figure shows a representative blood sample out of the four tested.

From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD33 Antibody, clone WM53 gallery image 4
Published customer image:
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived NK cells using immunoprecipitation.
Image caption:
CD33 expression and function in primary human NK cells. (d) CD33 coprecipitated SHP-2 phosphatase only in quiescent primary human NK cell lysates. The figure shows a representative experiment out of two with similar results.

From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD33 Antibody, clone WM53 gallery image 5
Published customer image:
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived quiescent NK cells by flow cytometry.
Image caption:
CD33 expression and function in primary human NK cells.CD33+ (WM53+ HIM3-4-) quiescent NK cells (R1 region, dark dots) were CD57-, NKG2D+, and partially expressed NKp46 and NKG2A antigens.

From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD33 Antibody, clone WM53 gallery image 6
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647) and SBV515 conjugated Mouse anti Human CD33 (MCA1271SBV515). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 7
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 8
Detection of recombinant Human CD33 with Mouse anti Human CD33 (MCA1271) in a Western blot analysis with Goat anti Mouse IgG:HRP (STAR207P) at a 1/10000 dilution as the secondary antibody. Different dilutions of MCA1271 are shown on lanes 2, 4, 6 and 8 at 1/200, 1/500, 1/1000 and 1/2000, respectively. Recombinant Human CD33 probed with STAR207P only at a 1/10000 dilution was included as a secondary only control (Lane 10).
Anti Human CD33 Antibody, clone WM53 gallery image 9
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 440 conjugated Mouse anti Human CD33 (MCA1271SBV440). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 10
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 610 conjugated Mouse anti Human CD33 (MCA1271SBV610). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 11
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647) and StarBright Violet 570 conjugated Mouse anti Human CD33 (MCA1271SBV570). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 12
Figure A. PE-Alexa Fluor 750 conjugated Mouse anti Human CD14 (MCA1568P750). Figure B. PE-Alexa Fluor 750 conjugated Mouse anti Human CD14 (MCA1568P750) and StarBright Violet 710 conjugated Mouse anti Human CD33 (MCA1271SBV710). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 13
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790). All experiments performed on red blood cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 14
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 475 conjugated Mouse anti Human CD33 (MCA1271SBV475). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 15
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and StarBright UltraViolet 400 conjugated Mouse anti Human CD33 (MCA1271SBUV400). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 16
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD14 (MCA1568SBUV510). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD14 (MCA1568SBUV510) and StarBright Violet 760 conjugated Mouse anti Human CD33 (MCA1271SBV760). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 17
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD14 (MCA1568SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD14 (MCA1568SBV475) and StarBright UltraViolet 665 conjugated Mouse anti Human CD33 (MCA1271SBUV665). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 18
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD14 (MCA1568SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD14 (MCA1568SBV710) and StarBright UltraViolet 445 conjugated Mouse anti Human CD33 (MCA1271SBUV445). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 19
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790) and StarBright UltraViolet 445 conjugated Mouse anti Human CD14 (MCA1568SBUV445). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 20
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and StarBright UltraViolet 795 conjugated Mouse anti Human CD33 (MCA1271SBUV795). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 21
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright UltraViolet 510 conjugated Mouse anti Human CD33 (MCA1271SBUV510). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 22
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790) and StarBright UltraViolet 510 conjugated Mouse anti Human CD14 (MCA1568SBUV510). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 23
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright UltraViolet 575 conjugated Mouse anti Human CD33 (MCA1271SBUV575). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 24
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright UltraViolet 605 conjugated Mouse anti Human CD33 (MCA1271SBUV605). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 25
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD33 (MCA1271SBV440). Figure B StarBright Violet 440 conjugated Mouse anti Human CD33 (MCA1271SBV440) and StarBright Blue 580 conjugated Mouse anti Human CD14 (MCA1568SBB580). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 26
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700). Figure B Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and StarBright Blue 580 conjugated Mouse anti Human CD33 (MCA1271SBB580). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 27
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Blue 615 conjugated Mouse anti Human CD33 (MCA1271SBB615). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 28
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Blue 675 conjugated Mouse anti Human CD33 (MCA1271SBB675). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 29
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD33 (MCA1271SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD33 (MCA1271SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD14 (MCA1568SBB675). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 30
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD14 (MCA1568SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD14 (MCA1568SBUV400) and StarBright Blue 765 conjugated Mouse anti Human CD33 (MCA1271SBB765). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 31
Figure A. StarBright Ultra Violet 665 conjugated Mouse anti Human CD33 (MCA1271SBUV665). Figure B. StarBright Ultra Violet 665 conjugated Mouse anti Human CD33 (MCA1271SBUV665) and StarBright Blue 810 conjugated Mouse anti Human CD14 (MCA1568SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 32
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Blue 810 conjugated Mouse anti Human CD33 (MCA1271SBB810). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 33
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD14 (MCA1568SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD14 (MCA1568SBUV795) and StarBright Yellow 575 conjugated Mouse anti Human CD33 (MCA1271SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 34
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670) and StarBright Yellow 575 conjugated Mouse anti Human CD14 (MCA1568SBY575). All experiments performed on red cell lysed Human blood gated on live single mononuclear cells, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 35
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Yellow 720 conjugated Mouse anti Human CD33 (MCA1271SBY720). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 36
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD33 (MCA1271SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD33 (MCA1271SBV610) and StarBright Yellow 720 conjugated Mouse anti Human CD14 (MCA1568SBY720). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 37
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Yellow 605 conjugated Mouse anti Human CD33 (MCA1271SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 38
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD33 (MCA1271SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD33 (MCA1271SBUV795) and StarBright Yellow 605 conjugated Mouse anti Human CD14 (MCA1568SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 39
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD33 (MCA1271SBV570). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD33 (MCA1271SBV570) and StarBright Yellow 800 conjugated Mouse anti Human CD14 (MCA1568SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 40
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515) and StarBright Yellow 800 conjugated Mouse anti Human CD33 (MCA1271SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 41
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670) and StarBright Yellow 665 conjugated Mouse anti Human CD19 (MCA1568SBY665). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 42
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Yellow 665 conjugated Mouse anti Human CD33 (MCA1271SBY665). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 43
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD33 (MCA1271SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD33 (MCA1271SBV515) and StarBright Red 775 conjugated Mouse anti Human CD14 (MCA1568SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD33 Antibody, clone WM53 gallery image 44
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515) and StarBright Red 775 conjugated Mouse anti Human CD33 (MCA1271SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD33

Product Type
Monoclonal Antibody
Clone
WM53
Isotype
IgG1
Specificity
CD33

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Product Code Applications Pack Size List Price Your Price Qty
MCA1271GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB 0.1 mg loader
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loader
MCA1271
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB 0.2 mg loader
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loader

Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.

Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Human AML cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 0.1 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Pack Size: 0.2 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells
Histology Positive Control Tissue
Lymphatic tissue

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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

Source Reference

  1. Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).
    Dis Markers. 5 (4): 215-25.

References for CD33 antibody

  1. Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
    Br J Haematol. 69 (2): 163-71.
  2. Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.
    Immunol Cell Biol. 71:571-81.
  3. Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.
    Exp Gerontol. 35: 213-24.
  4. Vamvakopoulos, J. et al. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
    Eur J Immunol. 32 (10): 2988-96.
  5. Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.
    BMC Cardiovasc Disord. 3: 6.
  6. Dahl C et al. (2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
    Allergy. 59 (10): 1087-96.
  7. Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.
    Eur J Immunol. 35: 243-51.
  8. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106 (10): 3567-74.
    9. View The Latest Product References
  9. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
    J Leukoc Biol. 79: 46-58.
  10. Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.
    Leuk Res. 31: 211-20.
  11. Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.
    Anal Chem. 84 (20): 8830-6.
  12. McCormack E et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
    Blood. 121 (7): e34-42.
  13. Hu, Z. et al. (2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody
    J Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.
  14. Hernández-Caselles T et al. (2019) CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
    J Immunol Res. 2019: 6032141.

RRID
AB_321662
UniProt
P20138
Entrez Gene
CD33
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation

MCA1271GA

148198

MCA1271

154525 1804

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