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CD326 antibody | VU-1D9

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Anti Human CD326 Antibody, clone VU-1D9 thumbnail image 1 Anti Human CD326 Antibody, clone VU-1D9 thumbnail image 2 Anti Human CD326 Antibody, clone VU-1D9 thumbnail image 3 Anti Human CD326 Antibody, clone VU-1D9 thumbnail image 4
Anti Human CD326 Antibody, clone VU-1D9 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD49f (MCA699A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD49f (MCA699A647) and FITC conjugated Mouse anti Human CD326 (MCA1870F). All experiments performed on human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD326 Antibody, clone VU-1D9 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse anti Human CD326 (MCA1870A647). All experiments performed on HT-29 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD326 Antibody, clone VU-1D9 gallery image 3
Published customer image:
Mouse anti Human CD326 antibody, cloneVU-1D9 (MCA1870G) used to identify CD326 expressing cells in circulating tumor cells by immunofluorescence.
Image caption:
Immunofluorescent micrographs of CTCs expressing bone alkaline phosphatase (B-ALP, or BAP), Epithelial cell adhesion molecular (EpCAM), DAPI, and CD45.
A. Expression of B-ALP and EpCAM in negative and positive control cell lines spiked into healthy volunteer blood. HELA cells positive for B-ALP (Green) and negative for EpCAM (Yellow) are shown on the left and T47D cells negative for B-ALP and positive for EpCAM are shown on the right. B. Changes in CTC B-ALP expression over time during radium-223 therapy. C. Examples of CTCs from men with bone metastatic CRPC treated with radium-223, demonstrating heterogeneity of CTC B-ALP expression in CTCs including in EpCAM negative CTCs. D. Plots of B-ALP (+) CTCs in healthy volunteers (n = 10) and patients (n = 20) over time, as well as Cellsearch CTCs (n = 20) from patients over time during radium-223 therapy (baseline, month 3 and month 6). *p = 0.0053 for difference in B-ALP (+) CTCs between healthy volunteers and mCRPC patients by non-parametric Kruskal-Wallis test.

From: Armstrong AJ, Gupta S, Healy P, Kemeny G, Leith B, Zalutsky MR, et al. (2019)
Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.
PLoS ONE 1 (5):e0216934.
https://doi.org/10.1371/journal.pone.0216934
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD326 Antibody, clone VU-1D9 gallery image 4
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD49f (MCA699A647) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD49f (MCA699A647) and PE conjugated Mouse anti Human CD326 (MC1870PE). All experiments performed on HT-29 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD326:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
VU-1D9
Isotype
IgG1
Specificity
CD326

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Product Code Applications Pack Size List Price Your Price Qty
MCA1870A647
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F 100 Tests/1ml loader
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Mouse anti Human CD326 antibody, clone VU-1D9 recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.

Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
HG9 cell line (small cell lung carcinoma)
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD326 antibody

  1. Koga, Y. et al. (2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.
    Gastroenterol Res Pract. 2008: 605273.
  2. Fillmore, C.M. et al. (2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.
    Proc Natl Acad Sci U S A. 107 (50): 21737-42.
  3. Murakata, A. et al. (2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.
    Ann Surg. 253: 94-100.
  4. Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
    Proc Natl Acad Sci U S A. 109 (8): 2772-7.
  5. Jeong, H.T. et al. (2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.
    Liver Int. 32: 430-40.
  6. Armstrong, A.J. et al. (2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.
    PLoS One. 14 (5): e0216934.

Further Reading

  1. Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
    Am J Pathol. 163 (6): 2139-48.

Flow Cytometry

Synonyms
Ep-CAM
UniProt
P16422
Entrez Gene
EPCAM
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0008284 positive regulation of cell proliferation
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016328 lateral plasma membrane

MCA1870A647

1808

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