CD324 antibody

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Mouse anti CD324

Mouse anti-human CD324 antibody recognizes cadherin-1, also known as CAM 120/80, E-cadherin, cell-CAM 120/80, epithelial cadherin or uvomorulin. This antibody has been extensively validated for western blotting using whole cell lysates.

CD324 Antibody with Control Lysate

Mouse anti-human CD324 antibody recognizes cadherin-1, also known as CAM 120/80, E-cadherin, cell-CAM 120/80, epithelial cadherin or uvomorulin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00502 WB 100 µl
VMA00502KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human CD324 antibody recognizes CD324, also known as cadherin-1, CAM 120/80, E-cadherin, epithelial cadherin or uvomorulin.

The CDH1 gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in CDH1 gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites (provided by RefSeq, Jul 2008).

Mouse anti Human CD324 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD324 detects a band of approximately 120 kDa in LNCaP cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to amino acids 338-621 of human CD324
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P12830 Related reagents
Entrez Gene
CDH1 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005913 cell-cell adherens junction
GO:0005925 focal adhesion
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0015629 actin cytoskeleton
GO:0016235 aggresome
GO:0016328 lateral plasma membrane
GO:0016342 catenin complex
GO:0016563 transcription activator activity
GO:0022408 negative regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043296 apical junction complex
GO:0045295 gamma-catenin binding
GO:0048471 perinuclear region of cytoplasm
GO:0050776 regulation of immune response
GO:0071285 cellular response to lithium ion
GO:0071681 cellular response to indole-3-methanol
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD324 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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