CD321 antibody | M.Ab.F11



Mouse anti Human CD321 antibody, clone F11 is able to activate platelets, leading to aggregation and granule secretion (Kornecki et al. 1990, Naik et al. 1995).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human platelet membranes.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y624
- Entrez Gene
- F11R
- GO Terms
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0006954 inflammatory response
- GO:0044419 interspecies interaction between organisms
- GO:0050900 leukocyte migration
- GO:0070830 tight junction assembly
- Regulatory
- For research purposes only
Applications of CD321 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 | 1/50 | |
Functional Assays 1 | |||
Immunofluorescence | |||
Immunoprecipitation | |||
Western Blotting | 1/100 | 1/1000 |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ml
- Western Blotting
- MCA2270 detects a band of approximately 35-40kDa in platelet cell lysates.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
Product Specific References
Source Reference
-
Kornecki, E. et al. (1990) Activation of human platelets by a stimulatory monoclonal antibody.
J Biol Chem. 265 (17): 10042-8.
References for CD321 antibody
-
Sobocka, M.B. et al. (2000) Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation.
Blood. 95 (8): 2600-9. -
Fraemohs, L. et al. (2004) The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain.
J Immunol. 173 (10): 6259-64. -
Immenschuh, S. et al. (2009) Transcriptional induction of junctional adhesion molecule-C gene expression in activated T cells.
J Leukoc Biol. 85 (5): 796-803. -
Haarmann, A. et al. (2010) Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
PLoS One. 5 (10): e13568.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra