CD321 antibody | M.Ab.F11

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Mouse anti Human CD321

Product Type
Monoclonal Antibody
Clone
M.Ab.F11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2270T F IF IP WB 25 µg
MCA2270 F FN* IF IP WB 0.2 mg
Mouse anti Human CD321 antibody, clone F11 recognises human Junctional adhesion molecule A, also known as CD321, JAM-1, JAM-A, Platelet F11 receptor, Platelet adhesion molecule 1 or PAM-1. CD321 is a 272 amino acid ~35 kDa single pass type 1 transmembrane glycoprotein expressed by endothelium, epithlial tissues and some leukocytes (Ostermann et al. 2002). CD321 was originally identified as the F11 receptor expressed on platelets (Kornecki et al. 1990).

Mouse anti Human CD321 antibody, clone F11 is able to activate platelets, leading to aggregation and granule secretion (Kornecki et al. 1990, Naik et al. 1995).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human platelet membranes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y624 Related reagents
Entrez Gene
F11R Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0006954 inflammatory response
GO:0044419 interspecies interaction between organisms
GO:0050900 leukocyte migration
GO:0070830 tight junction assembly
Regulatory
For research purposes only

Applications of CD321 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 Pack Size: 25 µg
1/10 Pack Size: 0.2 mg
1/50
Functional Assays 1
Immunofluorescence
Immunoprecipitation
Western Blotting 1/100 Pack Size: 25 µg
1/100 Pack Size: 0.2 mg
1/1000
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ml
Western Blotting
MCA2270 detects a band of approximately 35-40kDa in platelet cell lysates.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD321 antibody

  1. Kornecki, E. et al. (1990) Activation of human platelets by a stimulatory monoclonal antibody.
    J Biol Chem. 265 (17): 10042-8.
  2. Sobocka, M.B. et al. (2000) Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation.
    Blood. 95 (8): 2600-9.
  3. Immenschuh, S. et al. (2009) Transcriptional induction of junctional adhesion molecule-C gene expression in activated T cells.
    J Leukoc Biol. 85 (5): 796-803.
  4. Fraemohs, L. et al. (2004) The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain.
    J Immunol. 173 (10): 6259-64.
  5. Haarmann, A. et al. (2010) Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    PLoS One. 5 (10): e13568.