CD321 antibody | BV16

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Mouse anti Human CD321

Product Type: Monoclonal Antibody
Clone: BV16
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2269	C F			0.2 mg

Mouse anti Human CD321 antibody, clone BV16 recognizes human JAM-1, a ~40 kDa cell surface antigen found on the surface of platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 is also known as CD321.

For immunoprecipitation or western blotting of human JAM-1 we recommend the use of clone M.Ab.F11,

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Human JAM-Fc fusion protein.

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q9Y624 Related reagents
Entrez Gene: F11R Related reagents
GO Terms: GO:0007596 blood coagulation; GO:0016021 integral to membrane; GO:0005923 tight junction; GO:0006954 inflammatory response; GO:0044419 interspecies interaction between organisms; GO:0050900 leukocyte migration; GO:0070830 tight junction assembly
Regulatory: For research purposes only

Applications of CD321 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/10	1/50
Immunohistology - Frozen
Immunoprecipitation
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ml

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2b:FITC	HCA038F	0.1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG2b Negative Control	MCA691	100 Tests	F

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for CD321 antibody

Bazzoni, G. et al. (2000) Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin.
J Biol Chem. 275 (27): 20520-6.
Kasprowicz, V. et al. (2006) A highly restricted T-cell receptor dominates the CD8+ T-cell response to parvovirus B19 infection in HLA-A*2402-positive individuals.
J Virol. 80 (13): 6697-701.
Martinez-estrada, O.M. et al. (2001) Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial caco-2 cells.
J Biol Chem. 276 (12): 9291-6.

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