Mouse anti Human CD321 antibody, clone BV16
recognizes human JAM-1, a ~40 kDa cell surface antigen found on the surface of platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 is also known as CD321.
For immunoprecipitation or western blotting of human JAM-1 we recommend the use of clone M.Ab.F11
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Human JAM-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
integral to membrane
interspecies interaction between organisms
tight junction assembly
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD321 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ml
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD321 antibody
Bazzoni, G. et al. (2000) Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin.
J Biol Chem. 275 (27): 20520-6.
Kasprowicz, V. et al. (2006) A highly restricted T-cell receptor dominates the CD8+ T-cell response to parvovirus B19 infection in HLA-A*2402-positive individuals.
J Virol. 80 (13): 6697-701.
Martinez-estrada, O.M. et al. (2001) Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial caco-2 cells.
J Biol Chem. 276 (12): 9291-6.