CD321 antibody | BV16
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Mouse anti Human CD321 antibody, clone BV16 recognizes human JAM-1, a ~40 kDa cell surface antigen found on the surface of platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 is also known as CD321.
For immunoprecipitation or western blotting of human JAM-1 we recommend the use of clone M.Ab.F11,
For immunoprecipitation or western blotting of human JAM-1 we recommend the use of clone M.Ab.F11,
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human JAM-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y624
- Entrez Gene
- F11R
- GO Terms
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0006954 inflammatory response
- GO:0044419 interspecies interaction between organisms
- GO:0050900 leukocyte migration
- GO:0070830 tight junction assembly
- Regulatory
- For research purposes only
Applications of CD321 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ml
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
Product Specific References
References for CD321 antibody
-
Bazzoni, G. et al. (2000) Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin.
J Biol Chem. 275 (27): 20520-6. -
Kasprowicz, V. et al. (2006) A highly restricted T-cell receptor dominates the CD8+ T-cell response to parvovirus B19 infection in HLA-A*2402-positive individuals.
J Virol. 80 (13): 6697-701. -
Martinez-estrada, O.M. et al. (2001) Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial caco-2 cells.
J Biol Chem. 276 (12): 9291-6.
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