CD319 antibody | 162

Mouse anti Human CD319

Product Type
Monoclonal Antibody
Clone
162
Isotype
IgG2b
Specificity
CD319

Product Code Applications Pack Size List Price Your Price Qty
MCA4645GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.1 mg loader
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loader
MCA4645
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SDS Safety Datasheet SDS
F IP 0.2 mg loader
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loader
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Mouse anti Human CD319 antibody, clone 162 recognizes human CD319, otherwise known as CRACC (CD2-like receptor-activating cytotoxic cells), a type I transmembrane protein and member of the CD2 receptor family, expressed by natural killer (NK) cells, cytotoxic lymphocytes and activated B cells.

Unlike the CD2 family receptors 2B4 and NTB-A, which trigger NK cell-mediated cytotoxicity through the recruitment of the adaptor protein SAP (SLAM-associated protein); CD319 has been shown to activate cytotoxicity through a unique SAP-independent ERK-mediated signalling pathway, through association with, and subsequent phosphorylation by, the adaptor protein EAT-2.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
CD319 - HuIgG fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
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Description Mouse IgG2b Negative Control

References for CD319 antibody

  1. Bouchon, A. et al. (2001) Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family.
    J Immunol. 167 (10): 5517-21.
  2. Tassi, I. & Colonna, M. (2005) The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells.
    J Immunol. 175 (12): 7996-8002.
  3. Kawano, Y. et al. (2013) Hypoxia reduces CD138 expression and induces an immature and stem cell-like transcriptional program in myeloma cells.
    Int J Oncol. 43 (6): 1809-16.
  4. Pojero, F. et al. (2016) Utility of CD54, CD229, and CD319 for the identification of plasma cells in patients with clonal plasma cell diseases.
    Cytometry B Clin Cytom. 90 (1): 91-100.

Synonyms
CRACC
RRID
AB_1628806
UniProt
Q9NQ25
Entrez Gene
SLAMF7
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0042267 natural killer cell mediated cytotoxicity
GO:0030101 natural killer cell activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products