CD312 antibody | 2A1
Mouse anti Human CD312:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2A1
- Isotype
- IgG1
- Specificity
- CD312
Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Mouse anti Human CD312 antibody, clone 2A1 recognizes the stalk region of EMR2. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD312 antibody
-
Kwakkenbos, M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.
J Leukoc Biol. 71 (5): 854-62. -
Stacey, M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chondroitin sulfate glycosaminoglycans.
Blood. 102 (8): 2916-24. -
Lin, H.H. et al. (2004) Autocatalytic cleavage of the EMR2 receptor occurs at a conserved G protein-coupled receptor proteolytic site motif.
J Biol Chem. 279 (30): 31823-32. -
Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
FASEB J. 22 (3): 741-51. -
Tseng, W.Y. et al. (2013) Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases.
PLoS One. 8 (5): e63963. -
Huang, Y.S. et al. (2018) Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation.
Sci Rep. 8 (1): 4532. -
Shankar-Hari, M. et al. (2018) Early PREdiction of sepsis using leukocyte surface biomarkers: the ExPRES-sepsis cohort study.
Intensive Care Med. 44 (11): 1836-48. -
Bhudia, N. et al. (2020) G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody.
Sci Rep. 10 (1): 1004.
View The Latest Product References
Further Reading
-
Kwakkenbos, M.J. et al. (2004) The EGF-TM7 family: a postgenomic view.
Immunogenetics. 55 (10): 655-66.
- Synonyms
- EMR2
- RRID
- AB_566729
- UniProt
- Q9UHX3
- Entrez Gene
- EMR2
- GO Terms
- GO:0005886 plasma membrane
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0007218 neuropeptide signaling pathway
MCA2330PE
INN1608If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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