CD31 antibody | WM59
Filter by Application:F IF Reset
Mouse anti Human CD31:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Muller et al. 1989).CD31 is also known as PECAM-1.
The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
References for CD31 antibody
Cabañas, C. et al. (1989) Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.
Eur J Immunol. 19 (8): 1373-8.
Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts.
J Cell Biol. 128:1229-41.
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.
J Immunol. 145 (11): 3889-97.
DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.
Immunol Today. 15 (10): 490-5.
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62.
Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.
J Cell Biol. 148: 1151-8.
Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.
J Leukoc Biol. 82: 1278-88.
View The Latest Product References
Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.
Clin Immunol. 114: 154-63.
Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.
J Clin Pathol. 56 (10): 736-41.
Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.
BMC Cardiovasc Disord. Sep; 10:43.
Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
Clin Exp Immunol. 130: 256-62.
Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.
Anal Chem. 87 (6): 3239-46.
Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.
J Clin Pathol. 57 (9): 965-9.
Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
Tissue Eng Part C Methods. 21 (12): 1251-62.
Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.
Biomed Rep. 3 (5): 626-636.
Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.
Stem Cells Dev. 23 (22): 2730-43.
Fabre-Mersseman V et al. (2011) CD4⁺ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.
AIDS. 25 (9): 1153-62.
Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
Blood. 111 (10): 5173-81.
Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.
J Immunol. 173 (1): 230-5.
Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.
J Invest Dermatol. 135 (1): 130-41.
Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
Br J Haematol. 169 (4): 552-64.
Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.
Nat Commun. 6: 8009.
Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.
Am J Pathol. 185 (9): 2563-74.
Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.
Ann Neurol. 79 (2): 217-30.
Soh, B.S. et al. (2016) Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.
Nat Commun. 7: 10774.
GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244.
Duque, J.C. et al. (2019) Vascularization of the arteriovenous fistula wall and association with maturation outcomes.
J Vasc Access. : 1129729819863584. [Epub ahead of print]
Kim, J.S. et al. (2021) Randomization to Omega-3 Fatty Acid Supplementation and Endothelial Function in COPD: The COD-Fish Randomized Controlled Trial.
Chronic Obstr Pulm Dis. 8(1): 41-53.
Bye, A.P. et al. (2018) Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis.
Methods Mol Biol. 1812: 1-11.
Chai, S. et al. (2022) Identification of epithelial and mesenchymal circulating tumor cells in clonal lineage of an aggressive prostate cancer case.
NPJ Precis Oncol. 6 (1): 41.
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up