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CD305 antibody | NKTA255

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Anti Human CD305 Antibody, clone NKTA255 thumbnail image 1
Anti Human CD305 Antibody, clone NKTA255 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse anti Human CD305 (MCA2242A647). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD305:FITC

Product Type
Monoclonal Antibody
Clone
NKTA255
Isotype
IgG1
Specificity
CD305

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Product Code Applications Pack Size List Price Your Price Qty
MCA2242F
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SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Human CD305 antibody, clone NKTA255 recognizes human leukocyte-associated Ig-like receptor-1 (LAIR-1), a ~40 kDa cell surface molecule that functions as an inhibitory receptor on natural killer cells, monocytes and T cells. LAIR-1, otherwise known as CD305, is also expressed by other hematopoietic cells including B cells, but is not expressed by non-haematopoietic, platelets or erythrocytes.

Mouse anti Human CD305 antibody, clone NKTA255 is reported to inhibit NK cell triggering via CD16 molecules (Poggi et al. 1995).

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
NK cell lines B12.100 and AM.25
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3U1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Poggi, A. et al. (1995) p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans.
    Eur J Immunol. 25 (2): 369-76.

References for CD305 antibody

  1. Poggi, A. et al. (1997) p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes.
    Int Immunol. 9 (9): 1271-9.
  2. Poggi, A. et al. (2000) Engagement of the leukocyte-associated Ig-like receptor-1 induces programmed cell death and prevents NF-kappaB nuclear translocation in human myeloid leukemias.
    Eur J Immunol. 30 (10): 2751-8.
  3. Zocchi, M.R. et al. (2001) Leukocyte-associated Ig-like receptor-1 prevents granulocyte-monocyte colony stimulating factor-dependent proliferation and Akt1/PKB alpha activation in primary acute myeloid leukemia cells.
    Eur J Immunol. 31 (12): 3667-75.
  4. Poggi, A. et al. (2008) Lack of the leukocyte-associated Ig-like receptor-1 expression in high-risk chronic lymphocytic leukaemia results in the absence of a negative signal regulating kinase activation and cell division.
    Leukemia. 22 (5): 980-8.

Further Reading

  1. Meyaard, L. (2008) The inhibitory collagen receptor LAIR-1 (CD305).
    J Leukoc Biol. 83 (4): 799-803.

Synonyms
LAIR-1
RRID
AB_323554
UniProt
Q6GTX8
Entrez Gene
LAIR1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA2242F

1603 1808

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