CD30 antibody | D02-6E11

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Mouse anti Human CD30:Biotin

Product Type
Monoclonal Antibody
Clone
D02-6E11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6271B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CD30 antibody, clone D02-6E11, recognizes CD30 also known as Tumor Necrosis Factor Receptor Super Family member 8, CD30L receptor and Ki-1 antigen. CD30 is a 120 kDa transmembrane glycoprotein that belongs to the tumor necrosis factor receptor superfamily. CD30 is expressed on small subsets of activated T and B lymphocytes and a range of lymphoid neoplasms (Weyden et al. 2017).

Upon binding of its ligand CD30L, CD30 undergoes trimerization and recruits TNFR-associated factor (TRAF) and TRAF binding proteins generating a signaling complex which can then mediate MAPK and NFκB signaling (Weyden et al. 2017). In this way, CD30 signaling is able to regulate peripheral T cell responses, controls T cell survival, down regulates cytolitic capacity and regulates thymocyte survival (Muta & Podack 2013).

The biotinylated Mouse anti Human CD30 antibody, clone D02-6E11 (MCA6271B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD30 antibody, clone G05-2D2 (MCA6270GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CD30 (Phe19-Lys595)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P28908
Entrez Gene
TNFRSF8
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005737 cytoplasm
GO:0008285 negative regulation of cell proliferation
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0043065 positive regulation of apoptosis
GO:0045556 positive regulation of TRAIL biosynthetic process
Regulatory
For research purposes only

Applications of CD30 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CD30 MCA6270GA E 0.1 mg loader

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