CD30 antibody | Ber-H8

Mouse anti Human CD30

Product Type
Monoclonal Antibody
Clone
Ber-H8
Isotype
IgG1
Specificity
CD30

Product Code Applications Pack Size List Price Your Price Qty
MCA6134
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P 0.1 mg loader
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loader
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Mouse anti Human CD30, clone Ber-H8 recognizes CD30 also known as Ki-1 or TNFRSF8, a 120 kDa type I transmembrane glycoprotein receptor that belongs to the Tumor necrosis factor super family. CD30 is expressed by subpopulations of mitogen-stimulated T and B cell lymphoctyes and lymphoid neoplasms (Weyden et al. 2017).

CD30L, also known as TNSF8 or CD153 is the ligand of CD30. Once activated by its ligand, the receptor transduces the signal via the recruitment of TNFR-associated factor (TRAF) and TRAF associating proteins to form a signaling complex. This complex can subsequently activate NFκB and MAPK signaling pathways propagating anti-apoptotic/pro-survival responses (Muta and Podack 2013). CD30 has been shown to be highly expressed in Hodgkin’s lymphoma and anaplastic large cell lymphoma. Some research has also indicated that CD30 is associated with a range of non-hematopoetic pathologies, including germ cell tumors and testicular embryonal carcinomas (Weyden et al. 2017).

This clone Ber-H8 has been used in flow cytometry experiments to measure CD30 surface expression levels on CD4+ cells upon treatment of cord blood with human heat shock protein 60 (Aalbese et al. 2011).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD30 antibody

  1. Aalberse, J.A. et al. (2011) Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60).
    PLoS One. 6 (9): e24119.
  2. Muta, H. & Podack, E.R. (2013) CD30: from basic research to cancer therapy.
    Immunol Res. 57 (1-3): 151-8.
  3. VanDerWeyden, C.A. et al. (2017) Understanding CD30 biology and therapeutic targeting: a historical perspective providing insight into future directions.
    Blood Cancer J. 7 (9): e603.

UniProt
P28908
Entrez Gene
TNFRSF8
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005737 cytoplasm
GO:0008285 negative regulation of cell proliferation
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0043065 positive regulation of apoptosis
GO:0045556 positive regulation of TRAIL biosynthetic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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