CD30 antibody | Ber-H8
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Mouse anti Human CD30:FITC
Mouse anti Human CD30
Mouse anti Human CD30:RPE
- Product Type
- Monoclonal Antibody
- Clone
- Ber-H8
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6134F | F | 100 Tests/400µl |
|
|
| MCA6134 | C F P | 0.1 mg |
|
|
| MCA6134PE | F | 100 Tests/1ml |
|
Mouse anti Human CD30, clone Ber-H8 recognizes CD30 also known as Ki-1 or TNFRSF8, a 120 kDa type I transmembrane glycoprotein receptor that belongs to the Tumor necrosis factor super family. CD30 is expressed by subpopulations of mitogen-stimulated T and B cell lymphoctyes and lymphoid neoplasms (Weyden et al. 2017).
CD30L, also known as TNSF8 or CD153 is the ligand of CD30. Once activated by its ligand, the receptor transduces the signal via the recruitment of TNFR-associated factor (TRAF) and TRAF associating proteins to form a signaling complex. This complex can subsequently activate NFκB and MAPK signaling pathways propagating anti-apoptotic/pro-survival responses (Muta and Podack 2013). CD30 has been shown to be highly expressed in Hodgkin’s lymphoma and anaplastic large cell lymphoma. Some research has also indicated that CD30 is associated with a range of non-hematopoetic pathologies, including germ cell tumors and testicular embryonal carcinomas (Weyden et al. 2017).
This clone Ber-H8 has been used in flow cytometry experiments to measure CD30 surface expression levels on CD4+ cells upon treatment of cord blood with human heat shock protein 60 (Aalbese et al. 2011).
CD30L, also known as TNSF8 or CD153 is the ligand of CD30. Once activated by its ligand, the receptor transduces the signal via the recruitment of TNFR-associated factor (TRAF) and TRAF associating proteins to form a signaling complex. This complex can subsequently activate NFκB and MAPK signaling pathways propagating anti-apoptotic/pro-survival responses (Muta and Podack 2013). CD30 has been shown to be highly expressed in Hodgkin’s lymphoma and anaplastic large cell lymphoma. Some research has also indicated that CD30 is associated with a range of non-hematopoetic pathologies, including germ cell tumors and testicular embryonal carcinomas (Weyden et al. 2017).
This clone Ber-H8 has been used in flow cytometry experiments to measure CD30 surface expression levels on CD4+ cells upon treatment of cord blood with human heat shock protein 60 (Aalbese et al. 2011).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P28908
- Entrez Gene
- TNFRSF8
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005737 cytoplasm
- GO:0008285 negative regulation of cell proliferation
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0043065 positive regulation of apoptosis
- GO:0045556 positive regulation of TRAIL biosynthetic process
- Regulatory
- For research purposes only
Applications of CD30 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 4ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Product Specific References
References for CD30 antibody
-
Aalberse, J.A. et al. (2011) Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60).
PLoS One. 6 (9): e24119. -
Muta, H. & Podack, E.R. (2013) CD30: from basic research to cancer therapy.
Immunol Res. 57 (1-3): 151-8. -
VanDerWeyden, C.A. et al. (2017) Understanding CD30 biology and therapeutic targeting: a historical perspective providing insight into future directions.
Blood Cancer J. 7 (9): e603.
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