CD3/HLA2 | UCHT1 / WR18

Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE

Product Type
Dual Color Reagent
Clone
UCHT1 / WR18
Isotype
Cocktail
Specificity
CD3/HLA2

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DC019
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SDS Safety Datasheet SDS
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Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE Dual color reagent is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Clone UCHT1 recognizes the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (MOUSE)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control DC029 F 50 Tests loader
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Description Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

RRID
AB_321268
UniProt
P04234
P07766
P09693
Entrez Gene
CD3D
CD3E
CD3G
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
GO:0031295 T cell costimulation
GO:0045059 positive thymic T cell selection
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0006461 protein complex assembly
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0042110 T cell activation
View more products with CD3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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