CD3/HLA2 | UCHT1/WR18

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Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE

Product Type: Dual Color Reagent
Clone: UCHT1/WR18
Isotype: Cocktail

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
DC019	F			50 Tests

Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE Dual color reagent is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Clone UCHT1 recognizes the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.

Product Details

Target Species

Human

Product Form

Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised

Reconstitution

Reconstitute with 0.5 ml distilled water

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Antibody Isotypes

FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (MOUSE)

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P04234 Related reagents; P07766 Related reagents; P09693 Related reagents
Entrez Gene: CD3D Related reagents; CD3E Related reagents; CD3G Related reagents
GO Terms: GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005737 cytoplasm; GO:0031295 T cell costimulation; GO:0045059 positive thymic T cell selection; GO:0046982 protein heterodimerization activity; GO:0050852 T cell receptor signaling pathway; GO:0005057 receptor signaling protein activity; GO:0005887 integral to plasma membrane; GO:0007172 signal complex assembly; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0009897 external side of plasma membrane; GO:0017124 SH3 domain binding; GO:0019901 protein kinase binding; GO:0030159 receptor signaling complex scaffold activity; GO:0042608 T cell receptor binding; GO:0006461 protein complex assembly; GO:0007163 establishment or maintenance of cell polarity; GO:0015031 protein transport; GO:0042981 regulation of apoptosis; GO:0042110 T cell activation
Regulatory: For research purposes only

Applications of CD3/HLA2

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control	DC029	50 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

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