CD3/HLA2 | UCHT1 / WR18

ISO 9001 Quality Assurance Manufacturing | Print

No associated image currently available

Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE

Product Type: Dual Color Reagent
Clone: UCHT1 / WR18
Isotype: Cocktail
Specificity: CD3/HLA2

Quick Links:

Applications
Useful Reagents
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
DC019 Datasheet Datasheet SDS SDS	F	50 Tests		Log in	Get Quote
	Log in

Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE Dual color reagent is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Clone UCHT1 recognizes the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.

Target Species: Human
Product Form: Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution: Reconstitute with 0.5 ml distilled water
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Antibody Isotypes: FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (MOUSE)
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525

RPE 488nm laser 496 578

RPE 561nm laser 546 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
RPE 488nm laser	496	578
RPE 561nm laser	546	578

Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control	DC029	F	50 Tests	Log in	Get Quote
List Price	Your Price
	Log in
Description	Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control

Description	Product Code	Applications	Pack Size	Your Price
Human Seroblock	BUF070A	F	50 Test	Log in	Get Quote
List Price	Your Price
	Log in
Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test	Log in	Get Quote
List Price	Your Price
	Log in
Description	Human Seroblock

RRID: AB_321268
UniProt: P04234; P07766; P09693
Entrez Gene: CD3D; CD3E; CD3G
GO Terms: GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005737 cytoplasm; GO:0031295 T cell costimulation; GO:0045059 positive thymic T cell selection; GO:0046982 protein heterodimerization activity; GO:0050852 T cell receptor signaling pathway; GO:0005057 receptor signaling protein activity; GO:0005887 integral to plasma membrane; View More GO Terms; GO:0007172 signal complex assembly; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0009897 external side of plasma membrane; GO:0017124 SH3 domain binding; GO:0019901 protein kinase binding; GO:0030159 receptor signaling complex scaffold activity; GO:0042608 T cell receptor binding; GO:0006461 protein complex assembly; GO:0007163 establishment or maintenance of cell polarity; GO:0015031 protein transport; GO:0042981 regulation of apoptosis; GO:0042110 T cell activation