CD3/CD8/CD45/CD4 | UCHT1 / LT8 / F10-89-4 / RPA-T4

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Four-Color Human CD3/CD8/CD45/CD4 Flow Kit

Product Type
Four Color Reagent
Clone
UCHT1 / LT8 / F10-89-4 / RPA-T4
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4C008 F 50 Tests/0.5ml
Four-Color Human CD3/CD8/CD45/CD4 Flow Kit can be used for single-step identification of human mature T-cells (CD3+), helper/inducer (CD3+CD4+) T-cell subsets and cytotoxic (CD3+CD8+) T-cell subsets.

CD45 is a ~180-240kDa complex glycoprotein and member of the protein-tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), and is expressed on all haematopoietic cells with the exception of mature erythrocytes and platelets. CD45 is a complex molecule existing in a number of isoforms including CD45RA and CD45RO. Clone F10-89-4 reacts with all isoforms.

CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the &#a26;20kDa CD3ε chain.

CD4 is a single chain transmembrane glycoprotein and member of the immunoglobulin superfamily, which interacts directly with class II molecules of the major histocompatibility complex (MHCII), and is the primary receptor for the human immunodeficiency virus (HIV). CD4 is expressed by the helper/inducer (CD3+CD4+) T-cell subset, the majority of thymocytes, and at low levels by monocytes and tissue macrophages. The CD4 lymphocyte count provides an excellent indication of immune system health. Clone RPA-T4 binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen.

CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

Product Details

Target Species
Human
Product Form
Four color combination consisting of RPE-Cy5.5, APC, RPE and FITC conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

RPE-Cy5.5 conjugated CD3 (Mouse IgG1)
APC conjugated CD8 (Mouse IgG1)
RPE conjugated CD45 (Mouse IgG2a)
FITC conjugated CD4 (Mouse IgG1)
Reconstitution
Reconstitute with 0.5ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08575 Related reagents
P07766 Related reagents
P01732 Related reagents
P01730 Related reagents
Entrez Gene
CD4 Related reagents
CD8A Related reagents
CD3E Related reagents
PTPRC Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0019882 antigen processing and presentation
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
GO:0005057 receptor signaling protein activity
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD3/CD8/CD45/CD4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04B 100 Test/20ml F
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04C 500 Tests/100ml F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Flow Cytometry Absolute Count Standard™ FCSC580 20 Tests F

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