UCHT1 recognises the human CD3 antigen, which is expressed by all mature T cells.
RPA-T4 recognises the human CD4 antigen, a 56 kDa glycoprotein expressed by the helper-inducer subset of T cells. CD4 is also weakly expressed by monocytes.
DC002 may be used to simultaneously identify CD3 and CD4 positive cells, and to identify populations of cells that co-express these antigens.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
transmembrane receptor activity
transmembrane receptor protein tyrosine kinase signaling pathway
extracellular matrix structural constituent
endoplasmic reticulum membrane
induction by virus of host cell-cell fusion
zinc ion binding
initiation of viral infection
protein kinase binding
entry into host cell
T cell costimulation
maintenance of protein location in cell
T cell receptor complex
MHC class II protein binding
protein homodimerization activity
T cell selection
positive regulation of interleukin-2 biosynthetic process
positive regulation of protein kinase activity
regulation of defense response to virus by virus
T cell receptor signaling pathway
positive thymic T cell selection
protein heterodimerization activity
receptor signaling protein activity
integral to plasma membrane
signal complex assembly
G-protein coupled receptor protein signaling pathway
external side of plasma membrane
SH3 domain binding
receptor signaling complex scaffold activity
T cell receptor binding
protein complex assembly
establishment or maintenance of cell polarity
regulation of apoptosis
T cell activation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul to label 100ul whole blood.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for CD3/CD4
Beverley, P.C. & Callard, R.E. (1981) Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Eur J Immunol. 11 (4): 329-34.
Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
Adv Immunol. 44: 265-311.