CD3/CD4 | UCHT1/RPA-T4

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Mouse anti Human CD3:FITC/CD4:RPE

Product Type
Dual Color Reagent
Clone
UCHT1/RPA-T4
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC002 F 50 Tests
UCHT1 recognises the human CD3 antigen, which is expressed by all mature T cells.

RPA-T4 recognises the human CD4 antigen, a 56 kDa glycoprotein expressed by the helper-inducer subset of T cells. CD4 is also weakly expressed by monocytes.

DC002 may be used to simultaneously identify CD3 and CD4 positive cells, and to identify populations of cells that co-express these antigens.

Product Details

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P04234 Related reagents
P07766 Related reagents
P01730 Related reagents
P09693 Related reagents
Entrez Gene
CD4 Related reagents
CD3D Related reagents
CD3E Related reagents
CD3G Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0005737 cytoplasm
GO:0045059 positive thymic T cell selection
GO:0046982 protein heterodimerization activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0006461 protein complex assembly
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0042110 T cell activation
Regulatory
For research purposes only

Applications of CD3/CD4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 100ul whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC012 50 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD3/CD4

  1. Beverley, P.C. & Callard, R.E. (1981) Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
    Eur J Immunol. 11 (4): 329-34.
  2. Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
    Adv Immunol. 44: 265-311.