CD3/CD16 | UCHT1/ DJ130c

100% Secure

Mouse anti Human CD3:FITC/CD16:RPE

Product Type
Dual Color Reagent
Clone
UCHT1/ DJ130c
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC004 F 50 Tests
Mouse anti Human CD3:FITC/CD16:RPE dual color reagent has been developed to allow the simultaneous enumeration of Tcells and NK cells, as well as the subset of CD3+ve, CD16+ve lymphocytes.

CD3 is a complex cell-surface glycoprotein expressed by all mature T lymphocytes. This complex comprises five different polypeptide chains, with molecular weights of ~16-28 kDa. CD16 is a ~50-65 kDa glycoprotein expressed by NK cells, some T cells and granulocytes. It exists in two forms, a transmembrane protein expressed on NK cells and a GPI-linked form expressed on granulocytes. CD16 functions as a low affinity receptor for IgG, and is also known as FcRIII.

Product Details

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2a (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P04234 Related reagents
P09693 Related reagents
P07766 Related reagents
O75015 Related reagents
P08637 Related reagents
Entrez Gene
FCGR3B Related reagents
CD3D Related reagents
CD3E Related reagents
FCGR3A Related reagents
CD3G Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
GO:0031295 T cell costimulation
GO:0045059 positive thymic T cell selection
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0050776 regulation of immune response
GO:0006461 protein complex assembly
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0042110 T cell activation
Regulatory
For research purposes only

Applications of CD3/CD16

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 100ul whole blood.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD3/CD16

  1. Beverley, P.C.L. and Callard, R.E. (1981) Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
    Eur. J. Immunol. 11: 329-334.
  2. Edburg, J.C. et al. (1989) Human Fc gamma RIII (CD16). Isoforms with distinct allelic expression, extracellular domains, and membrane linkages on polymorphonuclear and natural killer cells.
    J. Immunol. 143: 1642-1649.
  3. Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.
    Eur. J. Immunol. 18: 705-710.