Mouse anti Human CD3:FITC/CD16:RPE dual color reagent has been developed to allow the simultaneous enumeration of Tcells and NK cells, as well as the subset of CD3+ve, CD16+ve lymphocytes.
CD3 is a complex cell-surface glycoprotein expressed by all mature T lymphocytes. This complex comprises five different polypeptide chains, with molecular weights of ~16-28 kDa. CD16 is a ~50-65 kDa glycoprotein expressed by NK cells, some T cells and granulocytes. It exists in two forms, a transmembrane protein expressed on NK cells and a GPI-linked form expressed on granulocytes. CD16 functions as a low affinity receptor for IgG, and is also known as FcRIII.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1.0%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG2a (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 6 months from date of despatch
- Entrez Gene
- GO Terms
anchored to membrane
integral to membrane
transmembrane receptor activity
T cell costimulation
positive thymic T cell selection
protein heterodimerization activity
T cell receptor signaling pathway
receptor signaling protein activity
integral to plasma membrane
signal complex assembly
G-protein coupled receptor protein signaling pathway
external side of plasma membrane
SH3 domain binding
protein kinase binding
receptor signaling complex scaffold activity
T cell receptor binding
regulation of immune response
protein complex assembly
establishment or maintenance of cell polarity
regulation of apoptosis
T cell activation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul to label 100ul whole blood
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Useful Reagents Available
Product Specific References
References for CD3/CD16
Beverley, P.C.L. and Callard, R.E. (1981) Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Eur. J. Immunol. 11: 329-334.
Edburg, J.C. et al. (1989) Human Fc gamma RIII (CD16). Isoforms with distinct allelic expression, extracellular domains, and membrane linkages on polymorphonuclear and natural killer cells.
J. Immunol. 143: 1642-1649.
Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.
Eur. J. Immunol. 18: 705-710.