Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit can be used for single-step identification of T-cells (CD3+), B-cells (CD3-CD19+) and NK cells (CD3-CD16+ and/or CD56+), useful in the study of NK-mediated cytotoxicity against malignant and virally infected cells and the characterization and monitoring of immunodeficiency and autoimmune diseases. CD45 is a 180-240 kDa complex glycoprotein and member of the protein-tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), and is expressed on all haematopoietic cells with the exception of mature erythrocytes and platelets. CD45 is a complex molecule existing in a number of isoforms including CD45RA and CD45RO. Clone F10-89-4 reacts with all isoforms. CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, θ), consisting of two heterodimers (εγ, εδ) and one homodimer (θθ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain. CD19 is a member of the immunoglobulin super family, which acts as a critical signal transduction molecule and regulator of B-cell development, differentiation and activation. CD19 is expressed on follicular dendritic cells and B-cells, from earliest recognizable B-lineage cells to B-cell blasts, but is lost on maturation to plasma cells. As such, CD19 is a commonly used pan B-cell marker. CD16/CD56, used in combination, facilitate the identification of natural killer (NK) cell sub-populations which express CD16+/CD56+, CD16+/CD56- or CD16-/CD56+ but lack CD3 and T-cell receptor (TCR) expression. There are important functional differences between these NK cell subsets. CD16-/CD56+(bright) cells, a minority in peripheral blood but representing the majority of NK cells in secondary lymphoid tissues, exhibit a high cytokine production capacity but are less effective at mediating antibody-dependent cellular cytotoxicity (ADCC) and natural NK cytotoxicity than other subsets.
- Target Species
- Product Form
- Four color combination consisting of PERCP, FITC, RPE and APC conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
PERCP conjugated CD3 (Mouse IgG1)
FITC conjugated CD16/CD56 (Mouse IgG1, IgG2a)
RPE conjugated CD45 (Mouse IgG2a)
APC conjugated CD19 (Mouse IgG1)
- Reconstitute with 0.5ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
| 5% || Sucrose |
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
anchored to membrane
transmembrane receptor activity
receptor signaling protein activity
integral to plasma membrane
signal complex assembly
G-protein coupled receptor protein signaling pathway
external side of plasma membrane
SH3 domain binding
protein kinase binding
receptor signaling complex scaffold activity
T cell costimulation
T cell receptor binding
protein heterodimerization activity
T cell receptor signaling pathway
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
immunoglobulin biosynthetic process
transmembrane receptor protein tyrosine phosphatase activity
negative regulation of protein kinase activity
positive regulation of B cell proliferation
regulation of S phase
B cell proliferation
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
integral to membrane
regulation of immune response
cellular defense response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD3/CD16/CD56/CD45/CD19
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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