CD3/CD16/CD56/CD45/CD19 | UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19

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Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit

Product Type
Four Color Reagent
Clone
UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4C007 F 50 Tests/0.5ml
Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit can be used for single-step identification of T-cells (CD3+), B-cells (CD3-CD19+) and NK cells (CD3-CD16+ and/or CD56+), useful in the study of NK-mediated cytotoxicity against malignant and virally infected cells and the characterization and monitoring of immunodeficiency and autoimmune diseases. CD45 is a 180-240 kDa complex glycoprotein and member of the protein-tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), and is expressed on all haematopoietic cells with the exception of mature erythrocytes and platelets. CD45 is a complex molecule existing in a number of isoforms including CD45RA and CD45RO. Clone F10-89-4 reacts with all isoforms. CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, θ), consisting of two heterodimers (εγ, εδ) and one homodimer (θθ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain. CD19 is a member of the immunoglobulin super family, which acts as a critical signal transduction molecule and regulator of B-cell development, differentiation and activation. CD19 is expressed on follicular dendritic cells and B-cells, from earliest recognizable B-lineage cells to B-cell blasts, but is lost on maturation to plasma cells. As such, CD19 is a commonly used pan B-cell marker. CD16/CD56, used in combination, facilitate the identification of natural killer (NK) cell sub-populations which express CD16+/CD56+, CD16+/CD56- or CD16-/CD56+ but lack CD3 and T-cell receptor (TCR) expression. There are important functional differences between these NK cell subsets. CD16-/CD56+(bright) cells, a minority in peripheral blood but representing the majority of NK cells in secondary lymphoid tissues, exhibit a high cytokine production capacity but are less effective at mediating antibody-dependent cellular cytotoxicity (ADCC) and natural NK cytotoxicity than other subsets.

Product Details

Target Species
Human
Product Form
Four color combination consisting of PERCP, FITC, RPE and APC conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

PERCP conjugated CD3 (Mouse IgG1)
FITC conjugated CD16/CD56 (Mouse IgG1, IgG2a)
RPE conjugated CD45 (Mouse IgG2a)
APC conjugated CD19 (Mouse IgG1)
Reconstitution
Reconstitute with 0.5ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5% Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08575 Related reagents
P07766 Related reagents
O75015 Related reagents
P08637 Related reagents
P13591 Related reagents
P15391 Related reagents
Entrez Gene
FCGR3B Related reagents
CD3E Related reagents
PTPRC Related reagents
FCGR3A Related reagents
NCAM1 Related reagents
CD19 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
GO:0005515 protein binding
GO:0006968 cellular defense response
Regulatory
For research purposes only

Applications of CD3/CD16/CD56/CD45/CD19

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04B 100 Test/20ml F
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04C 500 Tests/100ml F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Flow Cytometry Absolute Count Standard™ FCSC580 20 Tests F

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