CD3/CD16/CD56/CD45/CD19 | UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19
Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit can be used for single-step identification of T-cells (CD3+), B-cells (CD3-CD19+) and NK cells (CD3-CD16+ and/or CD56+), useful in the study of NK-mediated cytotoxicity against malignant and virally infected cells and the characterization and monitoring of immunodeficiency and autoimmune diseases. CD45 is a 180-240 kDa complex glycoprotein and member of the protein-tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), and is expressed on all haematopoietic cells with the exception of mature erythrocytes and platelets. CD45 is a complex molecule existing in a number of isoforms including CD45RA and CD45RO. Clone F10-89-4 reacts with all isoforms. CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, θ), consisting of two heterodimers (εγ, εδ) and one homodimer (θθ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain. CD19 is a member of the immunoglobulin super family, which acts as a critical signal transduction molecule and regulator of B-cell development, differentiation and activation. CD19 is expressed on follicular dendritic cells and B-cells, from earliest recognizable B-lineage cells to B-cell blasts, but is lost on maturation to plasma cells. As such, CD19 is a commonly used pan B-cell marker. CD16/CD56, used in combination, facilitate the identification of natural killer (NK) cell sub-populations which express CD16+/CD56+, CD16+/CD56- or CD16-/CD56+ but lack CD3 and T-cell receptor (TCR) expression. There are important functional differences between these NK cell subsets. CD16-/CD56+(bright) cells, a minority in peripheral blood but representing the majority of NK cells in secondary lymphoid tissues, exhibit a high cytokine production capacity but are less effective at mediating antibody-dependent cellular cytotoxicity (ADCC) and natural NK cytotoxicity than other subsets.
Product Details
- Target Species
- Human
- Product Form
- Four color combination consisting of PERCP, FITC, RPE and APC conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
PERCP conjugated CD3 (Mouse IgG1)
FITC conjugated CD16/CD56 (Mouse IgG1, IgG2a)
RPE conjugated CD45 (Mouse IgG2a)
APC conjugated CD19 (Mouse IgG1) - Reconstitution
- Reconstitute with 0.5ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08575
- P07766
- O75015
- P08637
- P13591
- P15391
- Entrez Gene
- FCGR3B
- CD3E
- PTPRC
- FCGR3A
- NCAM1
- CD19
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002378 immunoglobulin biosynthetic process
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007411 axon guidance
- GO:0030890 positive regulation of B cell proliferation
- GO:0033261 regulation of S phase
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
- GO:0005515 protein binding
- GO:0006968 cellular defense response
- Regulatory
- For research purposes only
Applications of CD3/CD16/CD56/CD45/CD19
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Erythrolyse Red Blood Cell Lysing Buffer (10x) | BUF04B | F | 100 Test/20ml |
|
|
| Erythrolyse Red Blood Cell Lysing Buffer (10x) | BUF04C | F | 500 Tests/100ml |
|
|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Flow Cytometry Absolute Count Standard™ | FCSC580 | F | 20 Tests |
