Rat anti Human CD3 antibody binds to an epitope within the highly conserved epsilon peptide chain, recognizing CD3 as a band of ~23 kDa in Jurkat cell lysates by western blotting under reducing conditions.
- Target Species
- Western Blotting
- Anti CD3 antibody detects a band of approximately 23 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide derived from the cytoplasmic epitope of human CD3
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P07766 Related reagents
- Entrez Gene
- CD3E Related reagents
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of CD3 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||C E P||0.5 mg|
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