CD3 antibody

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Western Blot Validated
Anti CD3 Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CD3 antibody (VMA00015) followed by detection with HRP conjugated Goat anti Rat IgG (1/50,000, STAR72) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to CD3 (molecular weight 23 kDa)

Rat anti CD3

CD3 antibody recognizes T cell surface glycoprotein CD3 epsilon chain, also known as T cell surface antigen T3/Leu-4 epsilon chain or CD3e. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00015
Datasheet
WB
MSDS
100 µl loader

Rat anti Human CD3 antibody recognizes human CD3, a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.

Rat anti Human CD3 antibody binds to an epitope within the highly conserved epsilon peptide chain, recognizing CD3 as a band of ~23 kDa in Jurkat cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti CD3 antibody detects a band of approximately 23 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide derived from the cytoplasmic epitope of human CD3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P07766 Related reagents
Entrez Gene
CD3E Related reagents
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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