Browsing Modes for Business Users

CD3 antibody | OKT3

Mouse anti Human CD3:Alexa Fluor® 488

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3ε), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3ε is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).

Mice that lack the CD3ε gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004).

Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Human peripheral blood lymphocytes
>90 % by SDS PAGE
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
For research purposes only
12 months from date of despatch.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 488 MCA929A488 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG2a Negative Control:Alexa Fluor® 488

Entrez Gene
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway


162996 163667 165383

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note