CD29 antibody | JB1B

100% Secure


Mouse anti Human CD29

Product Type
Monoclonal Antibody
Clone
JB1B
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1189 C E F IP WB 0.2 mg
Mouse anti Human CD29 antibody, clone JB1B recognizes human integrin beta1, also known as CD29, Fibronectin receptor subunit beta, Glycoprotein IIa or VLA-4 subunit beta. CD29 is a 798 amino acid ~130 kDa single pass type-1 transmembrane glycoprotein containing a single VWFA domain. Specificity for CD29 has been confirmed with integrin beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1 antibody.

Mouse anti Human CD29 antibody, clone JB1B has been successfully employed for the detection of porcine CD29 in formalin fixed, paraffin embedded material (Papageorgiou et al. 2014) and also to stimulate the adherence of several cell types (Wilkins et al. 1996).

The removal of azide is recommended prior to use in functional assays.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Cynomolgus monkey
Rhesus Monkey
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified Human beta1 integrin
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05556 Related reagents
Entrez Gene
ITGB1 Related reagents
Regulatory
For research purposes only

Applications of CD29 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1ug/ml 1ug/ml
Flow Cytometry 1ug/106cells
Immunohistology - Frozen 5ug/ml
Immunoprecipitation 1-5ug
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Application Based External Images

Western Blotting

Product Specific References

References for CD29 antibody

  1. Stupack, D.G. et al. (1994) Control of lymphocyte integrin function: evidence for multiple contributing factors.
    Cell Immunol. 155 (1): 237-45.
  2. Wilkins, J.A. et al. (1996) Control of beta1 integrin function. Localization of stimulatory epitopes.
    J Biol Chem. 271 (6): 3046-51.
  3. Yoshino N et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  4. Batista da Silva, A.P. et al. (2004) The major outer sheath protein of Treponema denticola inhibits the binding step of collagen phagocytosis in fibroblasts.
    Cell Microbiol. 6: 485-98.
  5. Levite, M. et al. (2000) Extracellular K(+) and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and beta1 integrins.
    J Exp Med. 191: 1167-76.
  6. Bass, R. et al. (2005) Regulation of urokinase receptor proteolytic function by the tetraspanin CD82.
    J Biol Chem. 280: 14811-8.
  7. Clark, K. et al. (2000) Production of recombinant soluble human integrin alpha4beta1.
    FEBS Lett. 471: 182-6.
  8. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.
    J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505.
  9. Takaki, T. (2006) Interferon-gamma inhibits collagen phagocytosis in human fibroblasts by inducing subcortical actin assembly and reducing ability of beta1 integrin to bind to collagen.
    Inflamm Res. 55: 534-42.
  10. Eirin A et al. (2017) Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells.
    PLoS One. 12 (3): e0174303.