CD289 antibody | 5G5
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine polynoprphonuclear leukocytes using flow cytometry.
Image caption:
Percentage of TLR positive PMNs in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represent the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <'0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine lymphocytes using flow cytometry.
Image caption:
Percentage of TLR positive lymphocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <0.005) BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine monocytes using flow cytometry.
Image caption:
Precentage of TLR positive monocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A.
Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD289 antibody, clone 5G5 recognizes human, canine and murine TLR9, otherwise known as CD289. TLR9 is a member of the Toll-like receptor (TLR) family and functions as a receptor for bacterial DNA (CpG-DNA), playing a fundamental role in pathogen recognition and activation of innate immunity.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
In mouse RAW264 cells TLR9 has been shown to be expressed intracellularly, but not at the cell surface.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
In mouse RAW264 cells TLR9 has been shown to be expressed intracellularly, but not at the cell surface.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Fusion protein of human TLR9 (amino acids 1-815).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NR96
- Entrez Gene
- TLR9
- GO Terms
- GO:0002237 response to molecule of bacterial origin
- GO:0042742 defense response to bacterium
- GO:0005764 lysosome
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0004888 transmembrane receptor activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005789 endoplasmic reticulum membrane
- GO:0006954 inflammatory response
- GO:0007252 I-kappaB phosphorylation
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0010008 endosome membrane
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0030277 maintenance of gastrointestinal epithelium
- GO:0032009 early phagosome
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032717 negative regulation of interleukin-8 production
- GO:0032722 positive regulation of chemokine production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032741 positive regulation of interleukin-18 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034122 negative regulation of toll-like receptor signaling pathway
- GO:0034123 positive regulation of toll-like receptor signaling pathway
- GO:0035197 siRNA binding
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043507 positive regulation of JUN kinase activity
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045087 innate immune response
- GO:0045356 positive regulation of interferon-alpha biosynthetic process
- GO:0045359 positive regulation of interferon-beta biosynthetic process
- GO:0046330 positive regulation of JNK cascade
- GO:0050729 positive regulation of inflammatory response
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
- Regulatory
- For research purposes only
Applications of CD289 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2265 detects a band of approximately 170-180 kDa in RAW264 cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for CD289 antibody
-
Ahmad-Nejad, P. et al. (2002) Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments.
Eur J Immunol. 32 (7): 1958-68. -
Batsford, S. et al. (2011) Protein level expression of Toll-like receptors 2, 4 and 9 in renal disease.
Nephrol Dial Transplant. 26 (4): 1413-6. -
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. -
Reineking, W. et al. (2018) Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands.
Vet Immunol Immunopathol. 206: 16-24.
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