CD289 antibody | 5G5
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine polynoprphonuclear leukocytes using flow cytometry.
Image caption:
Percentage of TLR positive PMNs in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represent the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <'0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine lymphocytes using flow cytometry.
Image caption:
Percentage of TLR positive lymphocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <0.005) BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD289 antibody, clone 5G5 (MCA2265) used for the evaluation of TLR9 expression in canine monocytes using flow cytometry.
Image caption:
Precentage of TLR positive monocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P <0.05; ** P <0.01; *** P <0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.
From: Maiolini A, Carlson R, Tipold A.
Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Human CD289
- Product Type
- Monoclonal Antibody
- Clone
- 5G5
- Isotype
- IgG2a
- Specificity
- CD289
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD289 antibody, clone 5G5 recognizes human, canine and murine TLR9, otherwise known as CD289. TLR9 is a member of the Toll-like receptor (TLR) family and functions as a receptor for bacterial DNA (CpG-DNA), playing a fundamental role in pathogen recognition and activation of innate immunity. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. In mouse RAW264 cells TLR9 has been shown to be expressed intracellularly, but not at the cell surface. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- < 0.1% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Fusion protein of human TLR9 (amino acids 1-815).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- Mouse anti Human CD289 antibody, clone 5G5 detects a band of approximately 170-180 kDa in RAW264 cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD289 antibody
-
Ahmad-Nejad, P. et al. (2002) Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments.
Eur J Immunol. 32 (7): 1958-68. -
Batsford, S. et al. (2011) Protein level expression of Toll-like receptors 2, 4 and 9 in renal disease.
Nephrol Dial Transplant. 26 (4): 1413-6. -
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. -
Reineking, W. et al. (2018) Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands.
Vet Immunol Immunopathol. 206: 16-24.
- Synonyms
- TLR9
- RRID
- AB_324147
- UniProt
- Q9NR96
- Entrez Gene
- TLR9
- GO Terms
- GO:0002237 response to molecule of bacterial origin
- GO:0042742 defense response to bacterium
- GO:0005764 lysosome
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0004888 transmembrane receptor activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005789 endoplasmic reticulum membrane
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0007252 I-kappaB phosphorylation
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0010008 endosome membrane
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0030277 maintenance of gastrointestinal epithelium
- GO:0032009 early phagosome
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032717 negative regulation of interleukin-8 production
- GO:0032722 positive regulation of chemokine production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032741 positive regulation of interleukin-18 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034122 negative regulation of toll-like receptor signaling pathway
- GO:0034123 positive regulation of toll-like receptor signaling pathway
- GO:0035197 siRNA binding
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043507 positive regulation of JUN kinase activity
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045087 innate immune response
- GO:0045356 positive regulation of interferon-alpha biosynthetic process
- GO:0045359 positive regulation of interferon-beta biosynthetic process
- GO:0046330 positive regulation of JNK cascade
- GO:0050729 positive regulation of inflammatory response
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
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