CD283 antibody | TLR3.7

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Mouse anti Human CD283

Product Type: Monoclonal Antibody
Clone: TLR3.7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2267GA	F* IP			0.1 mg
MCA2267	F* IP			0.2 mg

Mouse anti Human CD283 antibody, clone TLR3.7 recognizes human and canine TLR3, otherwise known as CD283. TLR3 is a member of the Toll-like receptor (TLR) family and functions as a receptor for double-stranded (ds) RNA.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

Cell surface expression of TLR3 has been reported on MRC-5 fibroblasts (Matsumoto et al.2002). Mouse anti Human CD283 antibody, clone TLR3.7 has been shown to suppress poly (I) : poly C mediated IFN beta production by these cells.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Dog

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Ba/F3 cells transfected with human TLR3 (CD283).

Approx. Protein Concentrations

Pack Size: 0.1 mg

IgG concentration 0.5mg/ml

Pack Size: 0.2 mg

IgG concentration 0.5 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: O15455 Related reagents
Entrez Gene: TLR3 Related reagents
GO Terms: GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003725 double-stranded RNA binding; GO:0042742 defense response to bacterium; GO:0004888 transmembrane receptor activity; GO:0005887 integral to plasma membrane; GO:0005789 endoplasmic reticulum membrane; GO:0006954 inflammatory response; GO:0006972 hyperosmotic response; GO:0007250 activation of NF-kappaB-inducing kinase activity; GO:0007252 I-kappaB phosphorylation; GO:0009597 detection of virus; GO:0010008 endosome membrane; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0032722 positive regulation of chemokine production; GO:0032728 positive regulation of interferon-beta production; GO:0032735 positive regulation of interleukin-12 production; GO:0032755 positive regulation of interleukin-6 production; GO:0032757 positive regulation of interleukin-8 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0034123 positive regulation of toll-like receptor signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045087 innate immune response; GO:0045356 positive regulation of interferon-alpha biosynthetic process; GO:0045359 positive regulation of interferon-beta biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0050729 positive regulation of inflammatory response; GO:0051607 defense response to virus
Regulatory: For research purposes only

Applications of CD283 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		1/10	1/50
Immunoprecipitation

¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Immunoprecipitation

Product Specific References

References for CD283 antibody

Matsumoto, M. et al. (2002) Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling.
Biochem Biophys Res Commun. 293 (5): 1364-9.
Tamassia, N. et al. (2008) Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils.
J Immunol. 181: 6563-73.
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226.
Mendoza, C. et al (2012) Toll-like receptor 3 activation affects serotonin transporter activity and expression in human enterocyte-like Caco-2 cells.
Cell Physiol Biochem. 30: 187-98.
Surendran, N. et al. (2018) TLR agonist combinations that stimulate Th type I polarizing responses from human neonates.
Innate Immun. 24 (4): 240-251.

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