CD282 antibody | TLR2.3
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F ResetMouse anti Human CD282
- Product Type
- Monoclonal Antibody
- Clone
- TLR2.3
- Isotype
- IgG2a
- Specificity
- CD282
Antibody Quality and Performance Guarantee
| Mouse anti Human CD282 antibody, clone TL2.3 recognizes human TLR2, otherwise known as CD282. TLR2 is a member of the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2. Mouse anti Human CD282 antibody, clone TL2.1 is reported to block TLR2 function. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- < 0.1% sodium azide (NaN3)
- Immunogen
- CHO cell line transfected with human TLR2 (CD282).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
- Histology Positive Control Tissue
- Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD282 antibody
-
Flo, T.H. et al. (2001) Differential expression of Toll-like receptor 2 in human cells.
J Leukoc Biol. 69 (3): 474-81. -
Yilmaz, A. et al. (2006) Differential effects of statins on relevant functions of human monocyte-derived dendritic cells.
J Leukoc Biol. 79 (3): 529-38. -
Lee, R.M. et al. (2006) Influenza a viruses upregulate neutrophil toll-like receptor 2 expression and function.
Scand J Immunol. 63 (2): 81-9. -
Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
Exp Hematol. 36 (9): 1167-75. -
Sels, J.W. et al. (2012) Fractional flow reserve is not associated with inflammatory markers in patients with stable coronary artery disease.
PLoS One. 7 (10): e46356. -
Jankovicova, K. et al. (2012) TLR2 in pleural fluid is modulated by talc particles during pleurodesis.
Clin Dev Immunol. 2012: 158287. -
Krejsek, J. et al. (2013) TLR2 and TLR4 expression on blood monocytes and granulocytes of cardiac surgical patients is not affected by the use of cardiopulmonary bypass.
Acta Medica (Hradec Kralove). 56 (2): 57-66. -
Krejsek, J. et al. (2013) TLR2 and TLR4 expression on blood monocytes and granulocytes of cardiac surgical patients is not affected by the use of cardiopulmonary bypass.
Acta Medica (Hradec Kralove). 56 (2): 57-66.
View The Latest Product References
-
Jaedicke, K.M. et al. (2013) Leptin up-regulates TLR2 in human monocytes.
J Leukoc Biol. 93 (4): 561-71. -
Al-Hassi, H.O. et al. (2014) Altered human gut dendritic cell properties in ulcerative colitis are reversed by Lactobacillus plantarum extracellular encrypted peptide STp.
Mol Nutr Food Res. 58 (5): 1132-43. -
Mann, E.R. et al. (2014) Human gut dendritic cells drive aberrant gut-specific t-cell responses in ulcerative colitis, characterized by increased IL-4 production and loss of IL-22 and IFNγ.
Inflamm Bowel Dis. 20 (12): 2299-307. -
Larsson, O. et al. (2018) Substance P represents a novel first-line defense mechanism in the nose.
J Allergy Clin Immunol. 141 (1): 128-136.e3.
Further Reading
-
Lien, E. et al. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products.
J Biol Chem. 274 (47): 33419-25.
- Synonyms
- TLR2
- RRID
- AB_931710
- UniProt
- O60603
- Entrez Gene
- TLR2
- GO Terms
- GO:0001875 lipopolysaccharide receptor activity
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005737 cytoplasm
- GO:0006917 induction of apoptosis
- GO:0006954 inflammatory response
- GO:0007252 I-kappaB phosphorylation
- GO:0008063 Toll signaling pathway
- View More GO Terms
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0030177 positive regulation of Wnt receptor signaling pathway
- GO:0032722 positive regulation of chemokine production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032741 positive regulation of interleukin-18 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034123 positive regulation of toll-like receptor signaling pathway
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0042495 detection of triacyl bacterial lipopeptide
- GO:0042496 detection of diacyl bacterial lipopeptide
- GO:0042497 triacyl lipopeptide binding
- GO:0042834 peptidoglycan binding
- GO:0045087 innate immune response
- GO:0046982 protein heterodimerization activity
- GO:0050729 positive regulation of inflammatory response
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051637 Gram-positive bacterial cell surface binding
- GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
- GO:0071223 cellular response to lipoteichoic acid
- GO:0071726 cellular response to diacyl bacterial lipopeptide
- GO:0071727 cellular response to triacyl bacterial lipopeptide
MCA2152GA
160373
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