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CD282 antibody | TLR2.3

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Anti Human CD282 Antibody, clone TLR2.3 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA596A647) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA596A647) and RPE conjugated Mouse anti Human CD282 (MCA2152PE). All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human CD282:FITC

Mouse anti Human CD282

Mouse anti Human CD282:RPE

Product Type
Monoclonal Antibody
Clone
TLR2.3
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2152F
Datasheet
F
SDS
0.1 mg loader

MCA2152GA
Datasheet
C F
SDS
0.1 mg loader

MCA2152PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Human CD282 antibody, clone TL2.3 recognizes human TLR2, otherwise known as CD282. TLR2 is a member of the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.

TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Mouse anti Human CD282 antibody, clone TL2.1 is reported to block TLR2 function.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
CHO cell line transfected with human TLR2 (CD282).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
O60603
Entrez Gene
TLR2
GO Terms
GO:0001875 lipopolysaccharide receptor activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0006917 induction of apoptosis
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0008063 Toll signaling pathway
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030177 positive regulation of Wnt receptor signaling pathway
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042496 detection of diacyl bacterial lipopeptide
GO:0042497 triacyl lipopeptide binding
GO:0042834 peptidoglycan binding
GO:0045087 innate immune response
GO:0046982 protein heterodimerization activity
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051637 Gram-positive bacterial cell surface binding
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0071223 cellular response to lipoteichoic acid
GO:0071726 cellular response to diacyl bacterial lipopeptide
GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD282 antibody

  1. Flo, T.H. et al. (2001) Differential expression of Toll-like receptor 2 in human cells.
    J Leukoc Biol. 69 (3): 474-81.
  2. Yilmaz, A. et al. (2006) Differential effects of statins on relevant functions of human monocyte-derived dendritic cells.
    J Leukoc Biol. 79 (3): 529-38.
  3. Lee, R.M. et al. (2006) Influenza a viruses upregulate neutrophil toll-like receptor 2 expression and function.
    Scand J Immunol. 63 (2): 81-9.
  4. Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
    Exp Hematol. 36 (9): 1167-75.
  5. Sels, J.W. et al. (2012) Fractional flow reserve is not associated with inflammatory markers in patients with stable coronary artery disease.
    PLoS One. 7 (10): e46356.
  6. Jankovicova, K. et al. (2012) TLR2 in pleural fluid is modulated by talc particles during pleurodesis.
    Clin Dev Immunol. 2012: 158287.
  7. Krejsek, J. et al. (2013) TLR2 and TLR4 expression on blood monocytes and granulocytes of cardiac surgical patients is not affected by the use of cardiopulmonary bypass.
    Acta Medica (Hradec Kralove). 56 (2): 57-66.
  8. Krejsek, J. et al. (2013) TLR2 and TLR4 expression on blood monocytes and granulocytes of cardiac surgical patients is not affected by the use of cardiopulmonary bypass.
    Acta Medica (Hradec Kralove). 56 (2): 57-66.
  9. Jaedicke, K.M. et al. (2013) Leptin up-regulates TLR2 in human monocytes.
    J Leukoc Biol. 93 (4): 561-71.
  10. Al-Hassi, H.O. et al. (2014) Altered human gut dendritic cell properties in ulcerative colitis are reversed by Lactobacillus plantarum extracellular encrypted peptide STp.
    Mol Nutr Food Res. 58 (5): 1132-43.
  11. Mann, E.R. et al. (2014) Human gut dendritic cells drive aberrant gut-specific t-cell responses in ulcerative colitis, characterized by increased IL-4 production and loss of IL-22 and IFNγ.
    Inflamm Bowel Dis. 20 (12): 2299-307.
  12. Larsson, O. et al. (2018) Substance P represents a novel first-line defense mechanism in the nose.
    J Allergy Clin Immunol. 141 (1): 128-136.e3.

Further Reading

  1. Lien, E. et al. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products.
    J Biol Chem. 274 (47): 33419-25.

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