CD282 antibody | TL2.1

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Mouse anti Human CD282

Product Type
Monoclonal Antibody
Clone
TL2.1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2151 C F FN* IP 0.2 mg
Mouse anti Human CD282 antibody, clone TL2.1 recognizes human TLR2, otherwise known as CD282. TLR2 is a member of the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Mouse anti Human CD282 antibody, clone TL2.1 is reported to block TLR2 function.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Marmoset
Cynomolgus monkey
Rhesus Monkey
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
CHO-TLR2 cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O60603 Related reagents
Entrez Gene
TLR2 Related reagents
GO Terms
GO:0001875 lipopolysaccharide receptor activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0006917 induction of apoptosis
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0008063 Toll signaling pathway
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030177 positive regulation of Wnt receptor signaling pathway
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042496 detection of diacyl bacterial lipopeptide
GO:0042497 triacyl lipopeptide binding
GO:0042834 peptidoglycan binding
GO:0045087 innate immune response
GO:0046982 protein heterodimerization activity
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051637 Gram-positive bacterial cell surface binding
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0071223 cellular response to lipoteichoic acid
GO:0071726 cellular response to diacyl bacterial lipopeptide
GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Functional Assays 1
Immunohistology - Frozen
Immunoprecipitation
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for CD282 antibody

  1. Lien, E. et al. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products.
    J Biol Chem. 274 (47): 33419-25.
  2. Flo, T.H. et al. (2001) Differential expression of Toll-like receptor 2 in human cells.
    J Leukoc Biol. 69 (3): 474-81.
  3. Bieback, K. et al. (2002) Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling.
    J Virol. 76: 8729-36.
  4. Karlsson, H. et al. (2004) Pattern of cytokine responses to gram-positive and gram-negative commensal bacteria is profoundly changed when monocytes differentiate into dendritic cells.
    Infect Immun. 72: 2671-8.
  5. Maguire, M. et al. (2005) Comparative cell signalling activity of ultrapure recombinant chaperonin 60 proteins from prokaryotes and eukaryotes.
    Immunology. 115: 231-8.
  6. Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
    Int Immunol. 19: 1271-9.
  7. Burgener, I.A. et al. (2008) Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
    Vet Immunol Immunopathol. 124: 184-91.
  8. Faure, E. et al. (2001) Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation
    J Immunol. 166: 2018-24.
  9. Hart, A.L. et al. (2005) Characteristics of intestinal dendritic cells in inflammatory bowel diseases.
    Gastroenterology. 129: 50-65.
  10. Melmed, G. et al. (2003) Human intestinal epithelial cells are broadly unresponsive to Toll-like receptor 2-dependent bacterial ligands: implications for host-microbial interactions in the gut.
    J Immunol. 170: 1406-15.
  11. Patel, D.N. et al. (2006) TLR4-NOX4-AP-1 signaling mediates lipopolysaccharide-induced CXCR6 expression in human aortic smooth muscle cells.
    Biochem Biophys Res Commun. 347: 1113-20.
  12. Lindsay, J.O. et al. (2005) Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease.
    Gut. 55: 348-55.
  13. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  14. Flo, T.H. et al. (2000) Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.
    J Immunol. 164 (4): 2064-9.
  15. Flo, T.H. et al. (2002) Involvement of toll-like receptor (TLR) 2 and TLR4 in cell activation by mannuronic acid polymers.
    J Biol Chem. 277 (38): 35489-95.
  16. Huang, D. et al. (2016) Hyperoxia induces inflammation and regulates cytokine production in alveolar epithelium through TLR2/4-NF-κB-dependent mechanism
    Eur Rev Med Pharmacol Sci. 20: 1399-410.