CD281 antibody | GD2.F4
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Mouse anti Human CD281 antibody, clone GD2.F4 recognizes human Toll-like receptor 1, also known as CD281or Toll/interleukin-1 receptor-like protein. CD281 is a 786 amino acid ~90 kDa single pass type 1 transmembrane glycoprotein protein expressed by peripheral blood leukocytes and in tissues such as, spleen and thymus.
CD281 plays an important role in the innate immune response to microbial pathogens (Netea et al. 2004).
Mouse anti Human CD281 antibody, clone GD2.F4 does not cross react with CD282 (TLR2), CD284 (TLR4) or TLR4/MD-2.
CD281 plays an important role in the innate immune response to microbial pathogens (Netea et al. 2004).
Mouse anti Human CD281 antibody, clone GD2.F4 does not cross react with CD282 (TLR2), CD284 (TLR4) or TLR4/MD-2.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- CD281 (TLR1) Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised mice where fused with cells of the NS0/1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q15399 Related reagents
- Entrez Gene
- TLR1 Related reagents
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006954 inflammatory response
- GO:0030670 phagocytic vesicle membrane
- GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
- GO:0042116 macrophage activation
- GO:0042495 detection of triacyl bacterial lipopeptide
- GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
- GO:0045087 innate immune response
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0046982 protein heterodimerization activity
- GO:0071727 cellular response to triacyl bacterial lipopeptide
- Regulatory
- For research purposes only
Applications of CD281 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD281 antibody
-
Wyllie, D.H. et al. (2000) Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses.
J Immunol. 165 (12): 7125-32. -
Uciechowski, P. et al. (2011) Susceptibility to tuberculosis is associated with TLR1 polymorphisms resulting in a lack of TLR1 cell surface expression.
J Leukoc Biol. 90 (2): 377-88. -
Visintin, A. et al. (2001) Regulation of Toll-like receptors in human monocytes and dendritic cells.
J Immunol. 166 (1): 249-55.
