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CD281 antibody | GD2.F4

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Mouse anti Human CD281

Product Type
Monoclonal Antibody
Clone
GD2.F4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2153GA
Datasheet
F
MSDS
0.1 mg loader

MCA2153
Datasheet
F
MSDS
0.2 mg loader

Mouse anti Human CD281 antibody, clone GD2.F4 recognizes human Toll-like receptor 1, also known as CD281 or Toll/interleukin-1 receptor-like protein. CD281 is a 786 amino acid ~90 kDa single pass type 1 transmembrane glycoprotein protein expressed by peripheral blood leukocytes and in tissues such as, spleen and thymus.

CD281 plays an important role in the innate immune response to microbial pathogens (Netea et al. 2004).

Mouse anti Human CD281 antibody, clone GD2.F4 does not cross react with CD282 (TLR2), CD284 (TLR4) or TLR4/MD-2.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
CD281 (TLR1) Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised mice where fused with cells of the NS0/1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q15399
Entrez Gene
TLR1
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006954 inflammatory response
GO:0030670 phagocytic vesicle membrane
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042116 macrophage activation
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0045087 innate immune response
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0046982 protein heterodimerization activity
GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD281 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

Source Reference

  1. Wyllie, D.H. et al. (2000) Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses.
    J Immunol. 165 (12): 7125-32.

References for CD281 antibody

  1. Visintin, A. et al. (2001) Regulation of Toll-like receptors in human monocytes and dendritic cells.
    J Immunol. 166 (1): 249-55.
  2. Klaffenbach, D. et al. (2005) Regulation and signal transduction of toll-like receptors in human chorioncarcinoma cell lines.
    Am J Reprod Immunol. 53 (2): 77-84.
  3. Uciechowski, P. et al. (2011) Susceptibility to tuberculosis is associated with TLR1 polymorphisms resulting in a lack of TLR1 cell surface expression.
    J Leukoc Biol. 90 (2): 377-88.
  4. Dreschler, K. et al. (2011) Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy.
    J Allergy Clin Immunol. 127 (2): 487-494.e1-3.
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