CD276 antibody | AbD11434

100% Secure


Human anti Human CD276

Product Type
Monoclonal Antibody
Clone
AbD11434
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA143 P* 0.1 mg
Human anti Human CD276 antibody, clone AbD11434 detects CD276, a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma.

Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing.

CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy(Kraan et al. 2014).

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with V5 and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human CD276-N1 fusion protein
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q5ZPR3 Related reagents
Entrez Gene
CD276 Related reagents
GO Terms
GO:0005102 receptor binding
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0009897 external side of plasma membrane
GO:0042102 positive regulation of T cell proliferation
GO:0042110 T cell activation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0050776 regulation of immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CD276 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Histology Positive Control Tissue
Human prostate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P