CD261 antibody | DR-4-02
Mouse anti Human CD261 antibody, clone DR-4-02 recognizes human death receptor 4 (DR4), also known as CD261 or TRAIL- R1. DR4 is a type I transmembrane protein of 468 amino acids, which is expressed in most human tissues including spleen, peripheral blood leucocytes, thymus and in a variety of tumour-derived cell lines.
DR4 plays a role in inducing cell death. The binding of TRAIL to DR4 triggers the activation of pro-caspases 8 and 10, leading to apoptosis.
DR4 plays a role in inducing cell death. The binding of TRAIL to DR4 triggers the activation of pro-caspases 8 and 10, leading to apoptosis.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Fusion protein containing the extracellular region of CD261 (DR4).
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized F1 hybrid mice were fuzed with cells of the SP2/0-Ag14 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O00220
- Entrez Gene
- TNFRSF10A
- GO Terms
- GO:0016021 integral to membrane
- GO:0005035 death receptor activity
- GO:0006919 activation of caspase activity
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0008134 transcription factor binding
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0008656 caspase activator activity
- GO:0045569 TRAIL binding
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD261 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD261 antibody
-
Cosgrove, C. et al. (2013) Early and nonreversible decrease of CD161++/MAIT cells in HIV infection.
Blood. 121: 951-61. -
Crescenzi, E. et al. (2011) NF-κB-dependent cytokine secretion controls Fas expression on chemotherapy-induced premature senescent tumor cells.
Oncogene. 30: 2707-17. -
Zhang, Y. and Zhang, B. (2008) TRAIL resistance of breast cancer cells is associated with constitutive endocytosis of death receptors 4 and 5.
Mol Cancer Res. 6: 1861-71.
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