CD258 antibody | D04-8A4
Mouse anti Human CD258
- Product Type
- Monoclonal Antibody
- Clone
- D04-8A4
- Isotype
- IgG2a
- Specificity
- CD258
Antibody Quality and Performance Guarantee
| Mouse anti Human CD258 antibody, clone D04-8A4, recognizes CD258 also known as TNFSF14, Herpes virus entry mediator and LIGHT. CD258 is the ligand of the receptor TNFRSF14, which is the cell surface receptor that the Herpes virus uses to attach to the cell surface and to enter into the cell, it is also a ligand of lymphotoxin-β receptor (Ware & Sedý 2011). Signaling events initiated upon binding of CD258 to these receptors is cell dependent. However, the responses are often related to immune system stimulation including the production of various cytokines, adhesion molecules that control the migration and positioning of dendritic cells and lymphocytes (Skeate et al. 2020, Tang et al. 2016). CD258 is primarily expressed by activated T cells, activated natural killer cells and immature dentritic cells. It is initially synthesized as a type II transmembrane protein, which can be proteolytically cleaved to release a soluble bioactive form (Ware & Sedý 2011). The purified Mouse anti Human CD258 antibody, clone D04-8A4 (MCA62680GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CD258 antibody, clone B01-9B3 (MCA6281B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human CD258 (Leu61-Val240)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human CD258:Biotin | MCA6281B | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Human CD258:Biotin | ||||||
- Synonyms
- TNFSF14
- UniProt
- O43557
- Entrez Gene
- TNFSF14
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006917 induction of apoptosis
- GO:0006955 immune response
- GO:0008588 release of cytoplasmic sequestered NF-kappaB
- View More GO Terms
- GO:0042098 T cell proliferation
- GO:0043027 caspase inhibitor activity
- GO:0043029 T cell homeostasis
MCA6280GA
100005376
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with TNFSF14 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up