CD257 antibody | T7-241

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Mouse anti Human CD257

Product Type
Monoclonal Antibody
Clone
T7-241
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4619T E F 25 µg
Mouse anti Human CD257 antibody, clone T7-241 recognizes human CD257 otherwise known as BAFF/BLyS. CD257 is a 285 amino acid type II transmembrane protein and member of the tumor necrosis factor (TNF) superfamily of ligands, which acts as a B and T cell stimulator and plays a role in the development of autoimmune diseases such as systemic lupus erythematosus.

CD257 is expressed by monocytes, macrophages, T cells and dendritic cells and acts through binding to CD267 (TACI), CD269 (BCMA) and CD268 (BAFFR) receptors, the latter interaction playing a critical role in the activation, survival, differentiation and proliferation of B cells.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human CD257.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of reconstitution.

More Information

UniProt
Q9Y275 Related reagents
Entrez Gene
TNFSF13B Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006955 immune response
GO:0008283 cell proliferation
Regulatory
For research purposes only

Applications of CD257 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD257 antibody

  1. Bossen, C. & Schneider, P. (2006) BAFF, APRIL and their receptors: structure, function and signaling.
    Semin Immunol. 18 (5): 263-75.