CD256 antibody | D05-1D4
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Mouse anti Human CD256
- Product Type
- Monoclonal Antibody
- Clone
- D05-1D4
- Isotype
- IgG2a
- Specificity
- CD256
Antibody Quality and Performance Guarantee
| Mouse anti Human CD256 antibody, clone D05-1D4 recognizes human CD256, also known as APRIL and TNFSF13. CD256, a 17 kDa soluble protein produced from the 32 kDa type II transmembrane protein by cleavage with furin, is a member of the tumor necrosis factor ligand superfamily (TNFLSF). CD256 and its receptor (TNFRSF17/BCMA) are both important for B-cell development (Langat et al. 2008) . It may also be implicated in the regulation of tumor cell growth and monocyte/macrophage-mediated immunological processes. CD256 is expressed in cancers of the colon, thyroid and lymphoid tissues and on monocytes and macrophages (Haiat et al. 2006). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from serum free tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant protein corresponding to aa 105-250 of human CD256
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Haiat, S. et al. (2006) Role of BAFF and APRIL in human B-cell chronic lymphocytic leukaemia.
Immunology. 118 (3): 281-92. -
Langat, D.L. et al. (2008) Signaling pathways for B cell-activating factor (BAFF) and a proliferation-inducing ligand (APRIL) in human placenta
Am J Pathol. 172:1303-11.
- Synonyms
- April
- UniProt
- O75888
- Entrez Gene
- TNFSF13
- GO Terms
- GO:0016020 membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005654 nucleoplasm
- GO:0006955 immune response
- GO:0008284 positive regulation of cell proliferation
- GO:0016071 mRNA metabolic process
- View More GO Terms
- GO:0048298 positive regulation of isotype switching to IgA isotypes
MCA5994GA
280814
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