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CD25 antibody | BC96

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Mouse anti Human CD25:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
BC96
Isotype
IgG1
Specificity
CD25

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Product Code Applications Pack Size List Price Your Price Qty
MCA2808C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse anti Human CD25 antibody, clone BC96 recognizes the human CD25 cell surface antigen, also known as the Interleukin-2 receptor subunit alpha or TAC antigen.

CD25 is derived from a 272 amino acid precursor to yield a 251 amino acid monomeric receptor of ~53kDa which acts as the low affinity receptor for IL-2. CD25 can also form a heterodimer with CD122 to form the high affinity receptor for IL-2. CD25 is expressed by activated lymphocytes (Jackson et al. 1990, Butcher et al. 1990). Defects in CD4+/ CD25+ regulatory T cells have been implicated in the pathogenesis of autoimmune diabetes (Lindley et al. 2005) and other autoimmune conditions (Sakaguchi et al. 1995).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to RPE-Cy5 - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE-Cy5 488nm laser 496 667
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.

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References for CD25 antibody

  1. Chapel, A. et al. (1992) Differential human immunodeficiency virus expression in CD4+ cloned lymphocytes: from viral latency to replication.
    J Virol. 66 (6): 3966-70.
  2. Ernst, C.W. et al. (2007) Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease.
    Clin Exp Immunol. 148 (2): 271-80.
  3. Barberá A et al. (2013) APL-1, an altered peptide ligand derived from human heat-shock protein 60, selectively induces apoptosis in activated CD4+ CD25+ T cells from peripheral blood of rheumatoid arthritis patients.
    Int Immunopharmacol. 17 (4): 1075-83.
  4. Duggleby, R.C. & Madrigal, J.A. (2014) Methods of detection of immune reconstitution and T regulatory cells by flow cytometry.
    Methods Mol Biol. 1109: 159-86.

Synonyms
IL-2R Alpha Chain
RRID
AB_1101641
UniProt
P01589
Entrez Gene
IL2RA
GO Terms
GO:0016021 integral to membrane
GO:0004911 interleukin-2 receptor activity
GO:0008283 cell proliferation

MCA2808C

171024 171129

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