CD243 antibody | OTI2C7

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Mouse anti CD243

Product Type
PrecisionAb Monoclonal
Clone
OTI2C7
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00324
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CD243 antibody recognizes the CD243 antigen, also known as multidrug resistance protein 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance and multidrug resistance protein 1 and ATP-binding cassette sub-family B member 1.

This membrane-associated protein, encoded by ABCB1 gene, is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by ABCB1 is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier (provided by RefSeq, Jul 2008).

Mouse anti Human CD243 antibody detects a band of 141 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD243 detects a band of approximately 141 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to amino acids 995-1280 of human CD243 (NP_000918) produced in SF9 cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P08183 Related reagents
Entrez Gene
ABCB1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0008559 xenobiotic-transporting ATPase activity
GO:0009986 cell surface
GO:0016324 apical plasma membrane
GO:0046581 intercellular canaliculus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD243 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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