CD23 antibody | 1B12

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Anti Human CD23 Antibody, clone 1B12 gallery image 1 — Staining of human tonsil. Formalin fixed, paraffin processed tissue. Mag. x 100 with Mouse anti human CD23 (**MCA1810T**).

Mouse anti Human CD23

Product Type: Monoclonal Antibody
Clone: 1B12
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1810T	C F P*			0.1 ml
MCA1810	C F P*			1 ml

Mouse anti Human CD23 antibody, clone 1B12 recognizes the human CD23 cell surface antigen also known as FcƐRII (Fc epsilon Receptor II). CD23 is a 45 kDa glycoprotein that functions as a low affinity receptor for IgE.

CD23 is expressed by peripheral B lymphocytes, cells of B cell chronic lymphocytic leukaemia and upon EBV transformed lymphoblastoid cells.

Product Details

Target Species

Human

Product Form

Tissue Culture Supernatant - liquid

Preservative Stabilisers

Pack Size: 0.1 ml

0.09%

Sodium Azide

Pack Size: 1 ml

0.09% Sodium Azide (NaN₃)

Immunogen

Recombinant human CD23.

Fusion Partners

Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line

Storage Information

Storage: Pack Size: 0.1 ml
Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 1 ml
Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: Please see label for expiry date

More Information

UniProt: P06734 Related reagents
Entrez Gene: FCER2 Related reagents
GO Terms: GO:0005576 extracellular region; GO:0004872 receptor activity; GO:0005887 integral to plasma membrane; GO:0005178 integrin binding; GO:0005529 sugar binding; GO:0019863 IgE binding; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0051712 positive regulation of killing of cells of other organism; GO:0051773 positive regulation of nitric-oxide synthase 2 biosynthetic process
Regulatory: For research purposes only

Applications of CD23 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen	1/20	1/40
Immunohistology - Paraffin ¹	1/20	1/40

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Tonsil

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for CD23 antibody

Watson, P. et al. (2000) Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma.
Histopathology. 36: 145-50.
Steiniger, B. et al. (2011) The follicular dendritic cell network in secondary follicles of human palatine tonsils and spleens.
Histochem Cell Biol. 135: 327-36.
Yoshida, S. et al. (2005) Primary breast diffuse large B-cell lymphoma shows a non-germinal center B-cell phenotype.
Mod Pathol. 18: 398-405.
Tasaki, K. et al. (2007) CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
Pathol Int. 57: 101-7.

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