CD23 antibody | 1B12

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Anti Human CD23 Antibody, clone 1B12 gallery image 1 — Staining of human tonsil. Formalin fixed, paraffin processed tissue. Mag. x 100 with Mouse anti human CD23 (**MCA1810T**).

Mouse anti Human CD23

Product Type: Monoclonal Antibody
Clone: 1B12
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1810 Datasheet	C F P* SDS	1 ml	Log in		Get Quote
MCA1810T Datasheet	C F P* SDS	0.1 ml	Log in		Get Quote

Mouse anti Human CD23 antibody, clone 1B12 recognizes the human CD23 cell surface antigen also known as FcƐRII (Fc epsilon Receptor II). CD23 is a 45 kDa glycoprotein that functions as a low affinity receptor for IgE.

CD23 is expressed by peripheral B lymphocytes, cells of B cell chronic lymphocytic leukaemia and upon EBV transformed lymphoblastoid cells.

Product Details

Target Species

Human

Product Form

Tissue Culture Supernatant - liquid

Preservative Stabilisers

Pack Size: 1 ml

0.09% Sodium Azide (NaN₃)

Pack Size: 0.1 ml

0.09%

Sodium Azide

Immunogen

Recombinant human CD23.

Fusion Partners

Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

UniProt: P06734
Entrez Gene: FCER2
GO Terms: GO:0005576 extracellular region; GO:0004872 receptor activity; GO:0005887 integral to plasma membrane; GO:0005178 integrin binding; GO:0005529 sugar binding; GO:0019863 IgE binding; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0051712 positive regulation of killing of cells of other organism; GO:0051773 positive regulation of nitric-oxide synthase 2 biosynthetic process
Regulatory: For research purposes only

Applications of CD23 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen	1/20	1/40
Immunohistology - Paraffin ¹	1/20	1/40

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Tonsil

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	F	100 Tests	Log in		Get Quote

Product Specific References

References for CD23 antibody

Watson, P. et al. (2000) Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma.
Histopathology. 36: 145-50.
Steiniger, B. et al. (2011) The follicular dendritic cell network in secondary follicles of human palatine tonsils and spleens.
Histochem Cell Biol. 135: 327-36.
Yoshida, S. et al. (2005) Primary breast diffuse large B-cell lymphoma shows a non-germinal center B-cell phenotype.
Mod Pathol. 18: 398-405.
Tasaki, K. et al. (2007) CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
Pathol Int. 57: 101-7.

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