CD23 antibody | 1B12

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Mouse anti Human CD23

Product Type
Monoclonal Antibody
Clone
1B12
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1810T
Datasheet
C F P*
MSDS
0.1 ml loader

MCA1810
Datasheet
C F P*
MSDS
1 ml loader

Mouse anti Human CD23 antibody, clone 1B12 recognizes the human CD23 cell surface antigen also known as FcƐRII (Fc epsilon Receptor II). CD23 is a 45 kDa glycoprotein that functions as a low affinity receptor for IgE.

CD23 is expressed by peripheral B lymphocytes, cells of B cell chronic lymphocytic leukaemia and upon EBV transformed lymphoblastoid cells.

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
Pack Size: 0.1 ml
0.09%Sodium Azide
Pack Size: 1 ml
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human CD23.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line

Storage Information

Storage
Pack Size: 0.1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 1 ml
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P06734 Related reagents
Entrez Gene
FCER2 Related reagents
GO Terms
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005529 sugar binding
GO:0019863 IgE binding
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051712 positive regulation of killing of cells of other organism
GO:0051773 positive regulation of nitric-oxide synthase 2 biosynthetic process
Regulatory
For research purposes only

Applications of CD23 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/20 1/40
Immunohistology - Paraffin 1 1/20 1/40
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD23 antibody

  1. Watson, P. et al. (2000) Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma.
    Histopathology. 36: 145-50.
  2. Steiniger, B. et al. (2011) The follicular dendritic cell network in secondary follicles of human palatine tonsils and spleens.
    Histochem Cell Biol. 135: 327-36.
  3. Yoshida, S. et al. (2005) Primary breast diffuse large B-cell lymphoma shows a non-germinal center B-cell phenotype.
    Mod Pathol. 18: 398-405.
  4. Tasaki, K. et al. (2007) CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
    Pathol Int. 57: 101-7.