CD227 antibody | C595 (NCRC48)

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Anti Human CD227 Antibody, clone C595 (NCRC48) thumbnail image 1

Anti Human CD227 Antibody, clone C595 (NCRC48) thumbnail image 2

Anti Human CD227 Antibody, clone C595 (NCRC48) thumbnail image 3

Anti Human CD227 Antibody, clone C595 (NCRC48) thumbnail image 4

Anti Human CD227 Antibody, clone C595 (NCRC48) gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD24 (MCA1379F). Figure B . FITC conjugated Mouse anti Human CD24 (MCA1379F) and Alexa Fluor 647 conjugated Mouse anti Human CD227 (MCA1742A647). All experiments performed on live single MCF-7 cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Anti Human CD227 Antibody, clone C595 (NCRC48) gallery image 2 — Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 (**MCA1742**) as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 (1695-0028) as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively

Anti Human CD227 Antibody, clone C595 (NCRC48) gallery image 3 — Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 (**MCA1742**) as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 (BioRad Catalog 22508250) as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.

Anti Human CD227 Antibody, clone C595 (NCRC48) gallery image 4 — Immunofluorescence staining of MCF-7 cells with Mouse anti Human CD227:FITC (**MCA1742F**) (green), nuclei are counterstained with DAPI (blue).

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Mouse anti Human CD227:Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: C595 (NCRC48)
Isotype: IgG3
Specificity: CD227

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References (9)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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MCA1742A647 Datasheet Datasheet SDS SDS	F	100 Tests/1ml		Log in	Get Quote
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Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.

Target Species: Human
Product Form: Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Urinary MUC-1 mucin.
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Alexa Fluor®647 650 665
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

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References for CD227 antibody

Price, M.R. et al. (1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.
Mol Immunol. 27 (8): 795-802.
Price, M.R. et al. (1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.
Br J Cancer. 61 (5): 681-6.
Price, M.R. et al. (1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.
J Immunol Methods. 139 (1): 83-90.
Denton, G. et al. (1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.
Eur J Cancer. 31A (2): 214-21.
Denton, G. et al. (1997) Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas.
Br J Cancer. 76 (5): 614-21.
Murray, A. et al (2001) Production and characterization of 188Re-C595 antibody for radioimmunotherapy of transitional cell bladder cancer.
J Nucl Med. 42: 726-32.
Hisatsune, A. et al. (2011) Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
Biochem Biophys Res Commun. 405: 377-81.
Ragupathi, G. et al. (2005) Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity.
J Immunol. 174: 5706-12.

View The Latest Product References

Huang, D.M. et al. (2004) Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells.
Prostate. 2004 May 15;59(3): 260-7.

Synonyms: CA 15-3; MUCIN 1
UniProt: P15941
Entrez Gene: MUC1