CD227 antibody | C595 (NCRC48)

Mouse anti Human CD227:Alexa Fluor® 647

Product Type
Monoclonal Antibody
C595 (NCRC48)

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Urinary MUC-1 mucin.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD227 antibody

  1. Price, M.R. et al. (1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.
    Mol Immunol. 27 (8): 795-802.
  2. Price, M.R. et al. (1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.
    Br J Cancer. 61 (5): 681-6.
  3. Price, M.R. et al. (1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.
    J Immunol Methods. 139 (1): 83-90.
  4. Denton, G. et al. (1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.
    Eur J Cancer. 31A (2): 214-21.
  5. Denton, G. et al. (1997) Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas.
    Br J Cancer. 76 (5): 614-21.
  6. Murray, A. et al (2001) Production and characterization of 188Re-C595 antibody for radioimmunotherapy of transitional cell bladder cancer.
    J Nucl Med. 42: 726-32.
  7. Hisatsune, A. et al. (2011) Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
    Biochem Biophys Res Commun. 405: 377-81.
  8. Ragupathi, G. et al. (2005) Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity.
    J Immunol. 174: 5706-12.
  9. View The Latest Product References
  10. Huang, D.M. et al. (2004) Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells.
    Prostate. 2004 May 15;59(3): 260-7.

CA 15-3
Entrez Gene



If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD227 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products