CD218a antibody | H44
Mouse anti Human CD218a antibody, clone H44 used to assess IL18Rα expression by flow cytometry and to inhibit IL-18Rα activity in a neutralizing assay.
Image caption:
Binding of soluble interleukin-18 receptor α complex to human natural killer cells expressing interleukin-18 receptor α.
(A) The reaction of the human interleukin (IL)-18 receptor α (hIL-18Rα) complex with the human natural killer (NK) cell line NK0 is shown. NK0 cells were stained with control biotin-labeled mouse immunoglobulin G1 (mIgG1-Bio), biotin-labeled anti-IL-18Rα monoclonal antibody clone 44 (H44-Bio) or biotin-labeled hIL-18Rα complex protein at 4°C for 30 minutes. For blocking assays (bottom), excess nonlabeled IL-18Rα complex proteins (80 μg) were preincubated with the cells at room temperature for 30 minutes. The cells were then stained with biotin hIL-18Rα complex at 4°C for 30 minutes. Streptavidin-PE was used for the second-step staining. FACS analysis was performed as described in Materials and methods. Percentages of immunopositive cells are shown in each graph. sIL-18Rα-Bio, biotin-labeled soluble interleukin-18 receptor α complex. (B) The neutralizing activity of hIL-18Rα complex protein against recombinant hIL-18 (rhIL-18) protein is shown. NK0 cells (5 × 105 cells/mL) were suspended in RPMI 1640 medium with 10% fetal calf serum and then pretreated with hIL-18Rα complex protein (0.003 to 10 μg/mL) for 15 minutes at room temperature. The cells were then stimulated with rhIL-2 (rhIL-2) (50 IU/mL) plus rhIL-18 (50 ng/mL) for 18 hours. Human interferon-γ in the supernatants was then analyzed using an enzyme-linked immunosorbent assay kit
From: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H.
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
Arthritis Res Ther. 2011 Mar 24;13(2):R52.
doi: 10.1186/ar3295.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD218a antibody, clone H44 used to assess IL18Rα expression and association with IL-18 by immunoprecipitation
Image caption:
Soluble interleukin-18 receptor α complex is associated with interleukin-18 and the soluble form of the interleukin-18 receptor β chain. (A) Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the serum interleukin (IL)-18 receptor α (IL-18Rα) complex. Purified H44 monoclonal antibody (mAb) was coupled to a 5-mL HiTrap NHS-activated HP column (GE Healthcare). Pooled human blood serum (120 mL) was applied to this affinity column. The IL-18Rα complex was eluted with elution buffer at a flow rate of 2 mL/minute. Every 1 mL of the elution buffer was collected into a test tube containing 50 mL of neutralization buffer (collected fractions were denoted in order as fractions 1, 2, 3 and so on). A 10-μL aliquot of every fraction (fractions 1 to 8) was treated with the same volume of sample buffer containing 4% SDS (Tris-Glycine SDS Sample Buffer (2×); Invitrogen). Electrophoresis was carried out in the presence of 0.1% SDS, and the gel was stained with Coomassie Brilliant Blue.
From: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H.
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
Arthritis Res Ther. 2011 Mar 24;13(2):R52.
doi: 10.1186/ar3295.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F FN ResetMouse anti Human CD218a
- Product Type
- Monoclonal Antibody
- Clone
- H44
- Isotype
- IgG1
- Specificity
- CD218a
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD218a recognizes CD218a also known as IL-18 receptor alpha chain. CD218a is a type I transmembrane protein and has a molecular weight of approxiamtely 75-80 kDa. CD218a expresssion has been detected on NK cells, neutrophils, endothelial cells and certain B and T cell subsets. CD218 combines with IL-18 receptor beta chain to form the IL-18 receptor. IL-18 signaling plays a vital role in innate and autoimmnue responses. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human NK cell line NK0 constitutively expressing IL-18 receptors
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/2 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Kitasato, Y. et al. (2004) Enhanced expression of interleukin-18 and its receptor in idiopathic pulmonary fibrosis.
Am J Respir Cell Mol Biol. 31 (6): 619-25.
Antibody Characterization Reference
-
Vermot-Desroches, C. et al. (2005) Monoclonal antibodies specific for the IL-18 receptor.
Cell Immunol. 236 (1-2): 101-4. Cell Immunol. 236 (1-2): 101-4.
References for CD218a antibody
-
Takei, S. et al. (2011) Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
Arthritis Res Ther. 13 (2): R52. -
Hoshino, T. et al. (2009) Role of proinflammatory cytokines IL-18 and IL-1beta in bleomycin-induced lung injury in humans and mice.
Am J Respir Cell Mol Biol. 41 (6): 661-70. -
Oda, H. et al. (2014) Interleukin-18 expression, CD8(+) T cells, and eosinophils in lungs of nonsmokers with fatal asthma.
Ann Allergy Asthma Immunol. 112 (1): 23-28.e1.
- Synonyms
- IL-18 Receptor
- UniProt
- Q13478
- Entrez Gene
- IL18R1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004908 interleukin-1 receptor activity
- GO:0045087 innate immune response
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