CD218a antibody | H44

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Mouse anti Human CD218a:FITC

Mouse anti Human CD218a

Mouse anti Human CD218a:RPE

Product Type: Monoclonal Antibody
Clone: H44
Isotype: IgG1

Product Code	Applications	Pack Size
MCA6062F	F	100 Tests/0.5ml
MCA6062GA	F	0.1 mg
MCA6062PE	F	100 Tests/0.5ml

Mouse anti Human CD218a recognizes CD218a also known as IL-18 receptor alpha chain. CD218a is a type I transmembrane protein and has a molecular weight of approxiamtely 75-80 kDa. CD218a expresssion has been detected on NK cells, neutrophils, endothelial cells and certain B and T cell subsets. CD218 combines with IL-18 receptor beta chain to form the IL-18 receptor. IL-18 signaling plays a vital role in innate and autoimmnue responses.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Immunogen: Human NK cell line NK0 constitutively expressing IL-18 receptors
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch
Shelf Life: 18 months from date of despatch
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q13478 Related reagents
Entrez Gene: IL18R1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0004908 interleukin-1 receptor activity; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of CD218a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry	1/2	1/10
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 1x10⁶ cells in 100μl
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 5μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

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