CD205 antibody | HD30
Mouse anti Human CD205 antibody, clone HD30 recognizes human Lymphocyte antigen 75, otherwise known as CD205, DEC205, C-type lectin domain family 13 member B or gp200-MR6. CD205 is a 1695 amino acid ~205 kDa single pass type I transmembrane glycoprotein and member of the macrophage mannose receptor family of C-type lectins, predominantly expressed by human dendritic cells.
CD205 is a recycled endocytic receptor involved in directing antigen uptake for processing and presentation by MHC class I. Multiple isoforms of human CD205, generated by alternative splicing have been described. Mouse anti Human CD205 antibody, clone HD30 recognizes the canonical isoform 4, reactivity with other isoforms has not been evaluated but both isoforms 2 and 3 contain most of the immunogen sequence used to generate clone HD30 making reactivity likely. However the antibody is not expected to be reactive with isoforms 1 and 5 which lack the immunogen sequence (UniProt : 060449).
CD205 is a recycled endocytic receptor involved in directing antigen uptake for processing and presentation by MHC class I. Multiple isoforms of human CD205, generated by alternative splicing have been described. Mouse anti Human CD205 antibody, clone HD30 recognizes the canonical isoform 4, reactivity with other isoforms has not been evaluated but both isoforms 2 and 3 contain most of the immunogen sequence used to generate clone HD30 making reactivity likely. However the antibody is not expected to be reactive with isoforms 1 and 5 which lack the immunogen sequence (UniProt : 060449).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Monkey Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Purified extracellular domain of human CD205-IgG1 Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- O60449 Related reagents
- Entrez Gene
- LY75 Related reagents
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0006897 endocytosis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of CD205 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/200 | |
| Immunohistology - Frozen | |||
| Western Blotting | |||
| Flow Cytometry | 1/10 | 1/200 | |
| Immunohistology - Frozen | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of suggested working dilution 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of suggested working dilution 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of suggested working dilution 106 cells in 100ul.
- Histology Positive Control Tissue
- Human or monkey spleen and lymph node.
- Histology Positive Control Tissue
- Human or monkey spleen and lymph node.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | 0.5 mg | C F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD205 antibody
-
Park, C.G. et al. (2012) Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals.
J Immunol Methods. 377 (1-2): 15-22.
