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CD203c antibody | NP4D6

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Anti Human CD203c Antibody, clone NP4D6 thumbnail image 1 Anti Human CD203c Antibody, clone NP4D6 thumbnail image 2 Anti Human CD203c Antibody, clone NP4D6 thumbnail image 3 Anti Human CD203c Antibody, clone NP4D6 thumbnail image 4
Anti Human CD203c Antibody, clone NP4D6 gallery image 1
Surface staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by Mouse anti Human CD203c:RPE (MCA6130PE)
Anti Human CD203c Antibody, clone NP4D6 gallery image 2
Surface staining of activated human basophil by FITC-conjugated anti Human CD63 (clone MEM-259, green) and Mouse anti Human CD203c:RPE (MCA6130PE, red); merged signal yellow. DNA visualized by DAPI (blue)
Anti Human CD203c Antibody, clone NP4D6 gallery image 3
Surface staining of human basophils in IgE-activated whole blood by Mouse anti Human CD203c:APC (MCA6130APC)
Anti Human CD203c Antibody, clone NP4D6 gallery image 4
Surface staining of human peripheral blood by Mouse anti Human CD203c:FITC (MCA6130F)

Mouse anti Human CD203c:APC

Mouse anti Human CD203c:FITC

Mouse anti Human CD203c

Mouse anti Human CD203c:RPE

Product Type
Monoclonal Antibody
Clone
NP4D6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6130APC
Datasheet
F
SDS
100 Tests/1ml loader

MCA6130F
Datasheet
F
SDS
100 Tests/400µl loader

MCA6130
Datasheet
F IC
SDS
0.1 mg loader

MCA6130PE
Datasheet
F
SDS
100 Tests/2ml loader

Mouse anti Human CD203c, clone NP4D6, recognizes CD203c. CD203c, also known as ENPP-3, is integral membrane hydrolase, that hydrolyzes extracellular nucleotide triphosphates into their monophosphate forms and plays a role in modulating purinergic signaling.

CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
HEK-293 cells transfected with human CD203c
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
O14638
Entrez Gene
ENPP3
GO Terms
GO:0003676 nucleic acid binding
GO:0004528 phosphodiesterase I activity
GO:0004551 nucleotide diphosphatase activity
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0006796 phosphate metabolic process
GO:0006955 immune response
GO:0009143 nucleoside triphosphate catabolic process
GO:0030247 polysaccharide binding
GO:0047429 nucleoside-triphosphate diphosphatase activity
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of CD203c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry 1/20 1/100
Immunocytochemistry
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 4ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 20ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC F 100 Tests loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Fluorescent Spectraviewer

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