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CD200R antibody | OX108

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Anti Human CD200R Antibody, clone OX108 thumbnail image 1 Anti Human CD200R Antibody, clone OX108 thumbnail image 2 Anti Human CD200R Antibody, clone OX108 thumbnail image 3 Anti Human CD200R Antibody, clone OX108 thumbnail image 4
Anti Human CD200R Antibody, clone OX108 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD4 (MCA1267F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD4 (MCA1267F) and Alexa Fluor 647 conjugated Mouse anti Human CD200R (MCA2282A647). All experiments performed on human blood gated on live, single lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD200R Antibody, clone OX108 gallery image 2
Published customer image:
Mouse anti Human CD200R antibody, clone OX-108 (MCA2282) used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
Image caption:
Double immunofluorescence stainings of CD68 and macrophage polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of CD68 (green) with MΦIFN-γ marker CD64 (A), MΦIL-4 markers CD200R (B) and CD14 (C), and MΦIL-10 markers CD163 (D), CD16 (E), and CD32 (F) (red) on synovial tissue macrophages. Figures are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients. Higher-magnification photos are included in each figure part.

From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL.
Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD200R Antibody, clone OX108 gallery image 3
Published customer image:
Mouse anti Human CD200R antibody, clone OX108 used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
Image caption:
Double-immunofluorescence stainings of MΦIFN-γ, MΦIL-4, and MΦIL-10 polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of MΦIFN-γ marker CD64 with CD200R (A), CD163 (B), and CD32 (C), colocalization of MΦIL-4 marker CD200R with CD14 (D), CD163 (E), and CD32 (F), and colocalization of MΦIL-10 marker CD163 with CD14 (G) and CD32 (H). Figure parts are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients.

From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL. Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.
Anti Human CD200R Antibody, clone OX108 gallery image 4
Published customer image:
AlexaFluor 647 conjugated Mouse anti Human CD200R antibody, clone OX-108 (MCA2282A647) used for the evaluation of CD200R expression on endometrial tissue by flow cytometry.
Image caption:
Endometrial Chlamydia infection promotes alternative activation of macrophages.
Endometrial tissue from women with no identified C. trachomatis, N. gonorrhoeae, or T. vaginalis lower or upper genital tract infection (n = 4), or from women with endocervical or endometrial C. trachomatis infection (n = 14 for Panel A; n = 12 for Panel B) were processed for flow cytometric analysis as described in Methods section. Macrophages were identified as FSC-AintSSC-AintCD45+CD15-CD14+HLA-DR+ live cells (as depicted in Figure S3), and 2 monoclonal antibody panels were used to interrogate macrophage differentiation and activation. Panel (A) evaluated expression of CD163, CD209, CD200R and CD206. Comparisons were done using unpaired one-tailed Student t-tests with Welch’s correction (horizontal bars indicate mean values for each group and significant p values are indicated in bold characters). Open circles indicate samples from uninfected controls; light gray circles indicate samples from women with cervical Chlamydia infection; and dark gray circles indicate samples from women with endometrial Chlamydia infection. Representative contour plots of CD200R, CD206, CD40 and CD80 expression by endometrial macrophages are displayed next to figures. For CD200R and CD206 expression evaluation (A), representative flow plots from peripheral blood monocytes treated with IL-4 (100 U/ml) for 24 hours and the corresponding untreated control are also shown.

From:Vicetti Miguel RD, Harvey SAK, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL (2013)
Human Female Genital Tract Infection by the Obligate Intracellular Bacterium Chlamydia trachomatis Elicits Robust Type 2 Immunity.
PLoS ONE 8(3): e58565.
doi: 10.1371/journal.pone.0058565
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human CD200R:RPE

Product Type
Monoclonal Antibody
Clone
OX108
Isotype
IgG1
Specificity
CD200R

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Product Code Applications Pack Size List Price Your Price Qty
MCA2282PET
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SDS Safety Datasheet SDS
F 25 Tests loader
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MCA2282PE
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F 100 Tests loader
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Mouse anti Human CD200R antibody, clone OX108 recognizes human CD200R, a cell surface glycoprotein (also known as OX2R). In humans CD200R is expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells, CD200-CD200R interaction may be involved in the control of myeloid cellular function (Wright et al. 2003).

Levels of expression on resting peripheral blood cells are relatively low.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
MCA2282PET: Reconstitute in 0.25 ml distilled water
MCA2282PE: Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Fusion protein hCD200RCD4d3+4.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD200R antibody

  1. Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
    J Immunol. 171 (6): 3034-46.
  2. Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
    J Neuropathol Exp Neurol. 68: 159-67.
  3. Melief, J. et al. (2012) Phenotyping primary human microglia: tight regulation of LPS responsiveness.
    Glia. 60 (10): 1506-17.
  4. Ambarus CA et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
    PLoS One. 7 (4): e35994.
  5. Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
    Arthritis Res Ther. 14 (2): R74.
  6. Vicetti, R.M. et al. (2013) Human female genital tract infection by the obligate intracellular bacterium
    Chlamydia trachomatis
    elicits robust Type 2 immunity.
    PLoS One. 8 (3): e58565.
  7. Melief, J. et al. (2013) Microglia in normal appearing white matter of multiple sclerosis are alerted but immunosuppressed.
    Glia. 61 (11): 1848-61.
  8. Vicetti Miguel, R.D. et al. (2013) Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity.
    PLoS One. 8 (3): e58565.
    9. View The Latest Product References
  9. Memarian, A. et al. (2013) Upregulation of CD200 is associated with Foxp3+ regulatory T cell expansion and disease progression in acute myeloid leukemia.
    Tumour Biol. 34 (1): 531-42.
  10. van de Garde, M.D. et al. (2014) Chronic exposure to glucocorticoids shapes gene expression and modulates innate and adaptive activation pathways in macrophages with distinct changes in leukocyte attraction.
    J Immunol. 192 (3): 1196-208.
  11. Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
    Breast Cancer Res. 17 (1): 101.
  12. Holmannova, D. et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
    Perfusion. 30 (7): 543-55.
  13. Wiedemann, G.M. et al. (2016) A novel TLR7 agonist reverses NK cell anergy and cures RMA-S lymphoma-bearing mice.
    Oncoimmunology. 5 (7): e1189051.
  14. Björnfot Holmström, S. et al. (2017) Gingival Tissue Inflammation Promotes Increased Matrix Metalloproteinase-12 Production by CD200Rlow Monocyte-Derived Cells in Periodontitis.
    J Immunol. 199 (12): 4023-35.
  15. Pascoal, M.R. et al. (2022) CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils.
    J Leukoc Biol. 111 (2): 367-77.

Synonyms
OX2R
RRID
AB_566627
UniProt
Q8TD46
Entrez Gene
CD200R1

MCA2282PET

1701

MCA2282PE

0812 1701

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